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Table 5 Loadings and principal component scores for 5 principal components obtained by principal component analysis a (PCA) of 11 variables from piglets fed diets with or without mycotoxin contamination, and with or without binder at d14 post-weaning

From: Mycotoxin binder improves growth rate in piglets associated with reduction of toll-like receptor-4 and increase of tight junction protein gene expression in gut mucosa

Treatmentb

Principal component

1

2

3

4

5

22.6%

22.2%

16.0%

15.6%

10.1%

CON

āˆ’0.225

0.119

0.119

0.719

0.517

CONĀ +Ā BIN

āˆ’0.019

0.006

0.390

āˆ’0.327

āˆ’0.455

DON

āˆ’0.571

0.630

āˆ’0.366

āˆ’0.477

0.119

DONĀ +Ā BIN

0.679

āˆ’0.630

āˆ’0.120

0.071

āˆ’0.151

P-value

ā€ƒMycotoxin

0.68

0.88

0.29

0.36

0.92

ā€ƒBinder

0.10

0.12

0.58

0.56

0.18

ā€ƒMycot. Ɨ Binder

0.23

0.19

0.98

0.08

0.44

BW d14c

Ā Ā 

0.889

Ā Ā 

FD4 permeability

Ā Ā Ā Ā 

0.974

OCLN

0.813

Ā Ā Ā Ā 

CLDN-1

0.767

Ā 

0.401

Ā Ā 

CLDN-2

0.343

Ā 

0.335

0.754

Ā 

CLDN-5

Ā 

0.546

0.713

Ā Ā 

TLR-4

Ā 

0.891

Ā Ā Ā 

TNF-Ī±

0.498

0.666

Ā Ā 

āˆ’0.300

IL-1Ī²

Ā 

0.866

Ā Ā Ā 

IL-8

Ā Ā Ā 

0.910

Ā 

IAP

0.856

Ā Ā Ā Ā 
  1. aRotation method: varimax with Kaiser normalisation; only correlations with |r|Ā >Ā 0.3 are indicated
  2. b CON negative control diet (uncontaminated basal diet), CONĀ +Ā BIN negative control diet with 1Ā kg/t mycotoxin binder, DON negative control diet with DON, DONĀ +Ā BIN negative control diet with DON and 1Ā kg/t mycotoxin binder. Principal components scores of subjects were analysed by General Linear model with fixed factor mycotoxin addition, binder addition and the interaction
  3. c BW body weight, CLDN-1 Claudin 1, CLDN-2 Claudin 2, CLDN-5 Claudin 5, FD4 FITC-dextran 4, IAP Intestinal alkaline phosphatase, IL-1Ī² Interleukin 1 beta, IL-8 Interleukin 8, OCLN Occludin, TLR-4 Toll like receptor 4, TNF-Ī± Tumor necrosis factor alpha