Mycotoxin binder improves growth rate in piglets associated with reduction of toll-like receptor-4 and increase of tight junction protein gene expression in gut mucosa

Table 2 Primer sequences used for reverse-transcription quantitative real-time PCR

Gene symbol^a	Accession number	Nucleotide sequence of primers, 5′-3’		Product length, bp	Tm, °C
Gene symbol^a	Accession number	Forward	Reverse	Product length, bp	Tm, °C
CLDN-1	NM_001244539.1	TATGACCCCATGACCCCAGT	GCAGCAAAGTAGGGCACCTC	108	59
CLDN-2	NM_001161638.1	TTCCTCCCTGTTCTCCCTGA	CACTCTTGGCTTTGGGTGGT	152	62
CLDN-5	NM_001161636.1	GTGGTCCGCGAGTTCTACGA	CTTGACAGGGAAGCCGAGGT	171	60
CLDN-7	NM_001160076.1	GGTCCCCACAAACGTGAAGTA	TCACTCCCAGGACAAGAGCA	114	60
HPRT-1	DQ178126	CCGAGGATTTGGAAAAGGT	CTATTTCTGTTCAGTGCTTTGATGT	181	60
IAP	XM_003133729.3	GGCCAACTACCAGACCATCG	CCGACTTCCCTGCTTTCTTG	116	60
IFN-γ	NM_213948.1	GCTTTTCAGCTTTGCGTGACT	CACTCTCCTCTTTCCAATTCTTCA	166	58
IL-1β	NM_214055.1	GCACCCAAAACCTGGACCT	CTGGGAGGAGGGATTCTTCA	143	58
IL-8	XM_003361958.3	TGTCAATGGAAAAGAGGTCTGC	CTGCTGTTGTTGTTGCTTCTCA	100	60
OCLD	NM_001163647.2	CATGGCTGCCTTCTGCTTCATTGC	ACCATCACACCCAGGATAGCACTCA	129	65
PPIA	NM_214353	CTGAAGCATACGGGTCCTGG	TGCCCTCTTTCACTTTGCCA	139	65
TBP	DQ178129	GATGGACGTTCGGTTTAGG	AGCAGCACAGTACGAGCAA	124	59
TLR-4	NM_001113039.2	TTCTTGCAGTGGGTCAAGGA	GACGGCCTCGCTTATCTGAC	135	58
TNF-α	NM_214022.1	CATGATCCGAGACGTGGAGC	AACCTCGAAGTGCAGTAGGC	151	62
ZO-1	XM_003480423.3	ATCTCGGAAAAGTGCCAGGA	CCCCTCAGAAACCCATACCA	172	61
ZO-2	XM_005660148.2	CCAGGAAGCACAGAATGCAA	AAGTCTGGCGGGACCTCTCT	148	61

^a CLDN-1 claudin-1, CLDN-2 claudin-2, CLDN-5 claudin-5, CLDN-7 claudin-7, HPRT-1 hypoxanthine phosphoribosyltransferase 1, IAP intestinal alkaline phosphatase, IFN-Ƴ interferon gamma, IL-1β interleukin 1 beta, IL-8 interleukin 8, OCLN occludin, PPIA peptidylprolyl isomerase A, TBP TATA-binding protein, TLR-4 toll-like receptor 4, TNF-α tumor necrosis factor alpha, ZO-1 zona occludens 1, ZO-2 zona occludens 2

ISSN: 2049-1891