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Table 1 Sequences of oligonucleotide primers used for real time-PCR

From: Plasma and cerebrospinal fluid interleukin-1β during lipopolysaccharide-induced systemic inflammation in ewes implanted or not with slow-release melatonin

GenBank Acc. No.

Gene

Amplicon size, bp

Temp. of primers annealing, °C

Forward/ reverse

Sequence

5′ → 3′

Reference

X54796.1

Il1B

137

59

forward

CAGCCGTGCAGTCAGTAAAA

[35]

reverse

GAAGCTCATGCAGAACACCA

NM_001206735.1

Il1r1

124

59

forward

GGGAAGGGTCCACCTGTAAC

[35]

reverse

ACAATGCTTTCCCCAACGTA

NM_001046210

Il1r2

161

59

forward

CGCCAGGCATACTCAGAAA

[36]

reverse

GAGAACGTGGCAGCTTCTTT

XM_004015970.1

Mmp3

112

60

forward

AAGGCAGACATTTTTGGCGG

Originally designed

reverse

ATGCCTCTTGGGGAACCTGC

XM_004014614.1

Mmp9

115

60

forward

CTTCCGATGGAAAGAACGGGC

Originally designed

reverse

GGGATCACAACGCCTTTGC

NM_001034034

Gapdh

143

60

forward

TGACCCCTTCATTGACCTTC

[27]

reverse

GATCTCGCTCCTGGAAGATG

NM_001009784.1

Actb

122

60

forward

GCCAACCGTGAGAAGATGAC

[27]

reverse

TCCATCACGATGCCAGTG

BC_108088.1

Hdac1

115

60

forward

CTGGGGACCTACGGGATATT

[35]

reverse

GACATGACCGGCTTGAAAAT