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Table 2 PCR primer sequences using for MeDIP

From: Negative effects of long-term feeding of high-grain diets to lactating goats on milk fat production and composition by regulating gene expression and DNA methylation in the mammary gland

Target genes MeDIP primer sequence GenBank accession No. PCR products, bp
FADS2 F:5′-GGCACTAATCCCAAGCAG-3’ XM_018043056.1 138
R:5′-GGAAACAATACAGGACCTCATA-3’   
ACSL1 F:5′-GGGAGGCGAGCAGAAAGA-3’ XM_018041881.1 142
R:5′-AACAGACGGTGGAGGGTG-3’   
ACSS1 F:5′-ACCTGAGAAGGGATGTGG-3’ XM_018057282.1 104
R:5′-AGAAGACTCAACGCAAACA-3’   
FAS F:5′-TTGCCTAAAGTCAGTGTCG-3’ NM_001285629.1 169
R:5′-GCAGGTCAACCGCATAAC-3’   
ACACA F:5′-GCTTTCTTCACCGAGGCT-3’ XM_018064168.1 173
R:5′-CGGAGGGTATCGCATTCA-3’   
ACSS2 F:5′-CCTCCCGTTCTGCTTTCC-3’ XM_005688483.3 132
R:5′-AGCCGTGCCTGGTGGTGTTG-3’   
SCD F:5′-CCCCAGTGCCCATCCATTT-3’ NM_001285619.1 168
R:5′-TCCCTTTCTCCTCGGCTTCTC-3’