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Table 1 PCR primer sequences of the target genes

From: Negative effects of long-term feeding of high-grain diets to lactating goats on milk fat production and composition by regulating gene expression and DNA methylation in the mammary gland

Target genes Primer sequences GenBank accession No. PCR products, bp
LPL F:5′-CAAGTCGCCTTTCTCCCGAT-3’ NM_001285607.1 177
R:5′-CTGCAATCACACGGAGAGCTT-3’   
ACACA F:5′-TATGACGGCAGCAGTTACACC-3’ XM_018064168.1 201
R:5′-CACCTCGATCTCAGCATAGCAC-3’   
FAS F:5′-CCCCAAGCTCTTTGACAACCG-3’ NM_001285629.1 100
R:5′-CAGCTCCTTGTACACGTCACC-3’   
FABP3 F:5′-TGAGACCACGGCAGATGA-3’ NM_001285701.1 230
R:5′-TAGCCCACTGGCAGAAGA-3’   
FABP4 F:5′-CATAAACTTAGATGAAGGTGCTC-3’ NM_001285623.1 116
R:5′-CACCGTTCATGACACATTCCA −3’   
DGAT1 F:5′-AAGCCCTTCAAGGACATG-3’ XM_018058728.1 100
R:5′-AGAGCCAGTAGAAGAAGATG-3’   
CPT1B F:5′- CCAGCCACAGTTCATCGGTA-3’ XM_018048994.1 103
R:5′-CAGTCTTCTCCTCGAACTCC-3’   
ACSS1 F:5′-TGAGCGACTGCGACTTCC-3’ XM_018057282.1 223
R:5′-CGGCGGACTCCATACCTCT-3’   
ACSS2 F:5′-TGAGCCAGAGGAAACCA-3’ XM_005688483.3 157
R:5′-ACACGCCAGCATAGCC-3’   
ACSL1 F:5′-CGCAGTGGCATCATTAG-3’ XM_018041881.1 183
R:5′-CTCGGTCTGTCCGTAGC-3’   
SREBP1 F:5′-ACGCCATCGAGAAACGCTAC-3’ NM_001285755.1 181
R:5′-GTGCGCAGACTCAGGTTCTC-3’   
PPARγ F:5′-CCTTCACCACCGTTGACTTCT-3’ NM_001285658.1 145
F:5′-GATACAGGCTCCACTTTGATTGC-3’   
CD36 F:5′-ATAATACTGCGGATGGA-3’ NM_001285578.1 165
R:5′-TCTCAACGAAAGGTGG-3’   
LDLR F:5′-GTGGCTGACACCAAAGGG-3’ XM_005682375.3 175
R:5′-CGGTCACCAGCGAGTAAAT-3’   
SCD F:5′-GTGGGTTGGCTGCTTG-3’ NM_001285619.1 243
R:5′-CCAGGTGGCATTGAGC-3’   
FADS2 F:5′-GCATCGCCTGGTTCACT-3’ XM_018043056.1 249
R:5′-GGAAGATGTTGGGTTTGG-3’   
GAPDH F:5′-GGGTCATCTCTCTGCACCT-3’ XM_005680968.3 176
R:5′-GGTCATAAGTCCCTCCACGA-3’