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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

Fig. 3

Purification of native OVGP1and its Western blot confirmation: (a) Lane 1: Total protein lysate, lane 2: Unbound fraction, lane 3: Molecular weight marker, lane 4: Eluted protein fraction from WGA affinity column, lane 5: Eluted protein fraction from Mono Q anion- exchange column representing three major protein bands (~60- ~95 kDa) for different glycosylated forms of native OVGP1. b Western blot confirmation, lane 1: Molecular weight marker, lane 2: Purified native OVGP1

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