Table 2 Relative mRNA expression of target genes in liver tissue at 14 to 28 d relative to diet initiation and after a 7-day washout period (35 d) in cows fed a controlled-energy diet (CON, 1.30 Mcal/kg; n = 7) or a moderate-energy diet (OVE, 1.60 Mcal/kg; n = 7). All cows received an intravenous injection of 2,4-thiazolidinedione (TZD) (+) for 2 wk after biopsy collection at 14 d after initiation of dietary treatments, with the additional 2 wk of study serving as a washout period
Gene | Diet | Day | Â | P-value | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
CON | OVE | SEM | 14 | 21 | 28 | 35 | SEM | Diet | TZD | |
TZD |  |  |  | − | + | + | − |  |  |  |
IRS1 | 1.03 | 1.02 | 0.03 | 1.04a | 0.99b | 1.03a | -c | 0.029 | 0.77 | 0.04 |
INSR | 1.12b | 1.02a | 0.05 | 1.06a | 1.04a | 1.11b | − | 0.04 | 0.02 | 0.03 |
ACOX1 | 1.03b | 0.96a | 0.02 | 1.01 | 0.98 | 1.00 | − | 0.02 | < 0.01 | 0.16 |
PPARD | 1.07b | 0.99a | 0.04 | 1.04 | 1.01 | 1.03 | − | 0.03 | 0.01 | 0.21 |