Fig. 5

Knocking down PACS-2 alleviates the metabolic decline in oocytes from obese mice. a The mRNA levels of Pacs-2 after PACS-2 knockdown. b The relative mRNA levels of Pacs-2. N = 90 per group across three replicates. c Western blotting showing PACS-2 levels after PACS-2 knockdown. d The relative protein levels of PACS-2. N = 300 per group across three replicates. e [Ca²⁺]_m after PACS-2 knockdown; scale bars, 50 μm. f Quantitative analysis of [Ca²⁺]_m. N = 30 per group across three replicates. g Quantitative analysis of the immunoblots shown in Fig. 4(i). N = 300 per group across three replicates. h ROS and GSH levels after PACS-2 knockdown; scale bars, 100 μm. i Quantitative analysis of ROS and GSH levels. N = 30 per group across three replicates. Graphs show means ± SEM. * P < 0.05, ** P < 0.01, CD vs. HFD; t-test