Fig. 1

HFD induced obesity leads to increased content of MAM in mouse oocytes. a Characteristic TEM images of oocytes from control or obese mice. Magnification, 30,000×; scale bars, 500 nm. The lower panels showed a higher magnification of the selected areas in the top panels. ER, endoplasmic reticulum; M, mitochondria; N, nucleus. b, c The length of the ER tethered to the mitochondria was normalized to the ER length (b) or mitochondrial circumference (c). N = 30 (five sections per mice, six mice per group) for each group across three replicates. d The subcellular structures of a larger area of oocytes from both groups are depicted. Magnification, 15,000×; scale bars, 1 μm. Graphs show means ± SEM. * P < 0.05, ** P < 0.01, CD vs. HFD; t-test