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Table 4 Effects of methionine on the activities of trypsin, lipase and amylase in the intestine, Na+/K+-ATPase, alkaline phosphatase (AKP), γ-glutamyl transpeptidase (γ-GT) and creatine kinase (CK) in the proximal intestine (PI), mid intestine (MI) and distal intestine (DI) of sub-adult grass carp1

From: The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella)

Met, g/kg diet

2.21

4.24

6.22

8.25

10.24

12.26

Trypsin, U/mg protein

170.42 ± 8.13a

235.75 ± 7.31b

298.06 ± 5.53e

275.83 ± 5.93d

265.32 ± 9.89c

263.54 ± 7.52c

Lipase, U/g protein

26.92 ± 1.58a

28.54 ± 2.21a

44.78 ± 4.82b

28.54 ± 2.59a

29.31 ± 3.12a

27.24 ± 2.28a

Amylase, U/mg protein

1.78 ± 0.09a

1.95 ± 0.08b

1.90 ± 0.06b

1.88 ± 0.09b

1.90 ± 0.03b

1.94 ± 0.04b

Na+/K+-ATPase, μmol pi/mg protein per hour

 PI

0.56 ± 0.03ab

0.56 ± 0.04ab

0.66 ± 0.06c

0.62 ± 0.04bc

0.74 ± 0.09d

0.51 ± 0.02a

 MI

0.52 ± 0.04bc

0.55 ± 0.05bcd

0.56 ± 0.04cd

0.59 ± 0.05d

0.50 ± 0.04b

0.44 ± 0.03a

 DI

0.41 ± 0.03a

0.43 ± 0.04ab

0.47 ± 0.05b

0.40 ± 0.04a

0.40 ± 0.04a

0.39 ± 0.04a

AKP, U/g protein

 PI

95.50 ± 10.36a

114.14 ± 8.49b

187.58 ± 20.75e

167.47 ± 13.52d

167.87 ± 14.96d

133.74 ± 13.56c

 MI

37.84 ± 4.02a

51.76 ± 5.44b

52.45 ± 4.17b

75.72 ± 7.24c

71.65 ± 7.42c

72.29 ± 8.36c

 DI

43.89 ± 2.68a

46.89 ± 2.70ab

48.87 ± 2.51bc

59.24 ± 3.37d

51.74 ± 3.38c

44.72 ± 3.75a

γ-GT, U/g protein

 PI

3.62 ± 0.18a

8.55 ± 0.28e

6.83 ± 0.36d

5.89 ± 0.36c

5.92 ± 0.24c

4.92 ± 0.18b

 MI

5.01 ± 0.39a

7.01 ± 0.22c

7.07 ± 0.40c

5.62 ± 0.32b

5.56 ± 0.22b

4.79 ± 0.16a

 DI

0.52 ± 0.04a

1.69 ± 0.14b

2.07 ± 0.13c

2.21 ± 0.17c

2.19 ± 0.13c

2.18 ± 0.16c

CK, U/mg protein

 PI

1.91 ± 0.21a

2.28 ± 0.19b

3.75 ± 0.25d

3.63 ± 0.37cd

3.41 ± 0.37c

2.17 ± 0.22ab

 MI

1.65 ± 0.13e

1.46 ± 0.14d

0.91 ± 0.09c

0.71 ± 0.05b

0.54 ± 0.04a

0.59 ± 0.07a

 DI

3.43 ± 0.18

3.36 ± 0.27

3.37 ± 0.17

3.24 ± 0.36

3.29 ± 0.34

3.27 ± 0.26

Regressions

 Y Trypsin = −2.768X2 + 47.626X + 84.338

R 2 = 0.878

P = 0.042

 Y Na + /K + -ATPase in MI = −0.004X2 + 0.047X + 0.428

R 2 = 0.893

P = 0.035

 Y AKP in PI = −2.452X2 + 40.210X + 10.672

R 2 = 0.822

P = 0.075

 Y AKP in MI = −0.378X2 + 9.100X + 18.654

R 2 = 0.886

P = 0.038

 Yγ-GT in DI = −0.033X2 + 0.622X – 0.561

R 2 = 0.953

P = 0.010

 YCK in PI = −0.065X2 + 1.011X – 0.264

R 2 = 0.852

P = 0.057

 YCK in MI = 0.012X2–0.291X + 2.313

R 2 = 0.962

P = 0.007

  1. a-eMeans in the same row with different superscript letters differ significantly (P < 0.05)
  2. 1Values are mean ± SD (n = 6)