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Table 5 Myofiber characteristics in the Semitendinosus muscle of selected offspring born from oviduct ligated (OL; non crowded) and intact (IN; relatively crowded) sows fed an unsupplemented (Ctrl) or l-arginine supplemented diet (ARG) from d 14 to 28 of gestation1

From: Impact of dietary l-arginine supply during early gestation on myofiber development in newborn pigs exposed to intra-uterine crowding

 

IN

OL

  

P-value2

 

Item

Ctrl

ARG

Ctrl

ARG

SEM

IUC

DIET

BtW

IUC×DIET

DIET×BtW

Observations

19

19

17

15

      

Muscle area, μm2 × 107

          

 Total

5.762

6.780

7.505

8.795

0.9429

0.144

0.003

0.653

0.738

0.053

 Dark portion

2.359x

2.278x

2.772ax

3.572by

0.2931

0.078

0.025

0.233

0.007

0.573

 Light portion

3.151

4.118

4.906

5.677

0.8279

0.143

0.011

0.910

0.786

0.061

Myofiber number, N

          

 Total muscle

476,025

527,711

536,451

607,928

35,511.0

0.236

0.003

0.136

0.604

0.475

 Dark portion

          

  Total myofibers

193,928

198,254

184,590

218,604

22,898.2

0.884

0.131

0.002

0.231

0.560

  Primary myofibers (P)

6,105

5,840

5,868

6,156

638.8

0.970

0.974

0.069

0.422

0.575

  Secondary myofibers (S)

187,823

192,414

178,722

212,448

22,455.9

0.883

0.124

0.002

0.231

0.559

  S:P ratio3

30.8

33.2

30.6

34.4

2.86

0.912

0.051

0.356

0.643

0.337

 Light portion

282,097

329,457

351,860

389,324

32,639.6

0.234

0.022

0.889

0.778

0.236

  1. 1Results are presented as least squares means of interactions between the two main factors extent of intra uterine crowding and dietary treatment and pooled SEM
  2. 2Probability values for the effects of extent of intra uterine crowding (IUC), dietary treatment (DIET), birth weight (BtW), and interactions IUC × DIET and DIET × BtW
  3. 3The secondary-to-primary myofiber ratio, calculated by dividing the number of secondary with the number of primary myofibers in the dark portion of the Semitendinosus muscle.
  4. a,bWithin a row for the main factor treatment, least squares means without a common superscript differ (P < 0.05)
  5. x,yWithin a row for the main factor treatment, least squares means without a common superscript differ (0.05 < P < 0.10)