Fig. 3

Distribution of peptides in inoculated and uninoculated feed. Marker: protein molecular weight markers (10–100 kDa); control: uninoculated mixed feed (corn 45%, soybean meal 45%, wheat bran 10%) was incubated at 37 °C for 72 h; B. subtilis: the mixed feed was inoculated with B. subtilis and fermented at 37 °C for 24 h; B. subtilis and E. faecium: first-stage fermented (B. subtilis) mixed feed was inoculated with E. faecium and fermented under anaerobic conditions at 37 °C for 48 h