Table 4 Differentially expressed proteins in the ileum mucosa of piglets supplemented by three lactobacilli in diets compared with antibiotics
Category | Spot No.a | Gene | Accession code | Protein | Expression change (Lactobacilli VS. Antibiotics) | Scoreb | Putative function |
|---|---|---|---|---|---|---|---|
Lactobacilli-insensitive spots compared with antibiotics | R1 | TPM2 | F1SG00 | Tropomyosin beta chain | Up-regulation | 261 | Stabilizing cytoskeleton actin filaments |
R2 | VIM | P02543 | Vimentin | Up-regulation | 138 | Maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions | |
R3 | KRT19 | F1S0J8 | Keratin type I cytoskeletal 19 | Up-regulation | 185 | Maintaining structural integrity of epithelial cells | |
R4 | TPM1 | F2Z5B6 | Tropomyosin alpha-1 chain | Up-regulation | 277 | Stabilizing cytoskeleton actin filaments | |
Lactobacilli-related spots compared with antibiotics | UB1(G1-1) | PIK3CG | O02697 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit | Only detectable in lactobacilli | 137 | Maintaining structural and functional integrity of epithelia |
UB2(G1-1) | CFL1 | P10668 | Cofilin-1 | Only detectable in lactobacilli | 121 | Regulation of cell morphology and cytoskeletal organization | |
UC1(G8-5) | ARHGDIB | F1SQW8 | Rho GDP-dissociation inhibitor 2 | Only detectable in lactobacilli | 109 | Small GTPase regulator activity receptor binding; | |
UC2(G8-5) | NPM1 | F1RRY2 | Nucleophosmin | Down-regulation | 99 | Ribosome biogenesis and transport | |
UD1(G22-2) | ARHGDIB | F1SQW8 | Rho GDP-dissociation inhibitor 2 | Only detectable in lactobacilli | 116 | Small GTPase regulator activity receptor binding; | |
UD2(G22-2) | ACTB | Q6QAQ1 | Actin cytoplasmic 1 | Only detectable in antibiotics | 139 | Involved in cell motility, structure, and integrity |