Fig. 5

Phylogeny of Methanomassiliicoccales sequences and highly abundant OTUs. Representative sequences of highly abundant OTUs (bold, ≥5% of the relative abundance in one of the ten samples) were aligned by MEGA 7. The GenBank accession numbers of these highly abundant OTUs are KX525649–61. Sixty-three sequences from Groups 8, 9, and 10 and isolates and enrichment cultures were selected in the RIM-DB. Phylogenetic analysis was performed on the 762 bp-to-1099 bp region of the 16S rRNA genes via the Maximum Likelihood method based on the Kimura 2-parameter model. The tree was resampled 500 times, and only bootstrap values of ≥70% are shown. The dendrogram was rooted with four Methanopyrus sequences. The scale bar indicates 0.02 inferred nucleotide substitutions per position. Mmc., Methanomassiliicoccus; Mmp., Methanomethylophilus