Alternative prediction methods of protein and energy evaluation of pig feeds

Table 7 In vitro filtration methods

Enzymes used in incubations
1	2	3	References
One-step incubation methods:
Pepsin			[77]
Trypsin			[78]
Papain			[79]
Pronase			[80]
Rennin			[81]
Duodenal digesta			[82]
Jejunal digesta			[82]
Feces extract			[82]
Two-step incubation methods:
Pepsin	Pancreatin		[10, 83, 84]
Pepsin	Pronase		[85]
Pepsin	Trypsin		[86]
Pepsin	Jejunal fluid		[87]
Three-step incubations methods:
Pepsin	Pancreatin	Cellulase	[93–95, 97, 98]
Pepsin	Pancreatin	Viscozyme	[96, 97]
Pepsin	Pancreatin	Rumen fluid	[99]

ISSN: 2049-1891