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Table 7 In vitro filtration methods

From: Alternative prediction methods of protein and energy evaluation of pig feeds

Enzymes used in incubations  
 1 2 3 References
One-step incubation methods:
 Pepsin    [77]
 Trypsin    [78]
 Papain    [79]
 Pronase    [80]
 Rennin    [81]
 Duodenal digesta    [82]
 Jejunal digesta    [82]
 Feces extract    [82]
Two-step incubation methods:
 Pepsin Pancreatin   [10, 83, 84]
 Pepsin Pronase   [85]
 Pepsin Trypsin   [86]
 Pepsin Jejunal fluid   [87]
Three-step incubations methods:
 Pepsin Pancreatin Cellulase [9395, 97, 98]
 Pepsin Pancreatin Viscozyme [96, 97]
 Pepsin Pancreatin Rumen fluid [99]