Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

Table 3 Analyzed chemical composition and Bacillus cell numbers in assay diets

	High-protein		Low-protein
Item	-	+	-	+
Dry matter, %	88.6	88.7	88.3	88.6
Crude protein, % DM	20.6	20.3	15.2	16.1
Ash, % DM	6.1	6.0	5.2	5.3
Ether extract, % DM	3.7	3.6	3.2	3.3
Neutral detergent fiber, % DM	12.7	13.1	10.1	10.5
Acid detergent fiber, % DM	7.0	6.6	5.4	5.1
Acid detergent lignin, % DM	1.1	0.9	0.8	0.8
Indispensable amino acids, % DM
Arginine	1.26	1.25	0.93	0.99
Histidine	0.46	0.46	0.35	0.36
Isoleucine	0.80	0.80	0.61	0.63
Leucine	1.53	1.53	1.15	1.19
Lysine	1.48	1.50	1.12	1.12
Methionine	0.50	0.51	0.38	0.37
Phenylalanine	0.95	0.95	0.69	0.74
Threonine	0.91	0.91	0.68	0.70
Tryptophan	0.27	0.27	0.20	0.21
Valine	1.02	1.01	0.76	0.79
Dispensable amino acids, % DM
Alanine	0.80	0.79	0.60	0.63
Aspartic acid	1.73	1.71	1.29	1.37
Cystine	0.33	0.33	0.25	0.26
Glutamic acid	4.08	4.03	3.05	3.20
Glycine	0.81	0.80	0.61	0.64
Proline	1.32	1.31	0.99	1.04
Serine	0.93	0.91	0.69	0.73
Bacillus cell numbers, CFU/kg feed
Bacillus subtilis	0.022 × 10⁹	0.860 × 10⁹	0.038 × 10⁹	0.970 × 10⁹
Bacillus licheniformis	<0.002 × 10⁹	0.680 × 10⁹	0.006 × 10⁹	0.570 × 10⁹

ISSN: 2049-1891