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Table 1 Ingredient composition of assay diets, % as-fed basis

From: Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

Item High-protein Low-protein
Barley 20.00 15.00
Wheat 51.00 38.24
Soybean meal 21.51 16.13
Oila 1.50 1.50
Cornstarchb 2.08 25.55
Vitamins and minerals premixc 0.76 0.76
Sodium chloride 0.07 0.07
Monocalcium phosphate 0.66 0.66
Calcium carbonate 0.65 0.65
Vitamin Ed 0.03 0.03
L-Lysine-HCle 0.61 0.46
DL-Methioninee 0.22 0.16
L-Isoleucinee 0.03 0.02
L-Leucinee 0.13 0.10
L-Threoninee 0.22 0.17
L-Tryptophane 0.01 0.01
L-Valinee 0.12 0.09
Titanium dioxide 0.40 0.40
Bacillus spp.f - -
Calculated chemical compositiong
 Metabolizable energy, MJ/kg 13.43 14.25
 Crude protein, % 18.00 14.00
 Calcium, % 0.66 0.63
 Available Phosphorus, % 0.27 0.25
 SIDg Lysine, % 1.20 0.92
 SIDg Methionine, % 0.43 0.33
 SIDg Threonine, % 0.73 0.56
  1. aBlend of rapeseed oil (75%) and soybean oil (25%)
  2. bRoquette, Lestrem, France
  3. cVilomin® 18950, Deutsche VilomixTierernährung GmbH, Neuenkirchen-Vörden, Germany; provided the following quantities of minerals and vitamins per kg of diet: Ca, 1.86 g; P, 0.38 g; Na, 0.42 g; Mg, 76.00 mg; Fe, 30.40 mg (FeSO4·H2O); Cu, 3.80 mg (CuSO4·5H2O); Mn, 20.29 mg (MnO); Zn, 25.38 mg (ZnO); I, 0.51 mg (Ca(IO3)2); Se, 0.10 mg (Na2SeO3); Co, 0.06 mg (2CoCO3·3Co(OH)2·H2O); vitamin A, 3,040 IU; vitamin D3, 456 IU; vitamin E, 19.00 mg; vitamin B1, 0.38 mg; vitamin B2, 1.18 mg; vitamin B6, 0.95 mg; vitamin B12, 7.60 μg; vitamin K3, 0.76 mg; niacin, 4.75 mg; calcium pantothenate, 2.85 mg; folic acid, 0.19 mg; choline chloride, 57.00 mg
  4. dLutavitE 50, BASF, Ludwigshafen, Germany
  5. eAll crystalline amino acids (AA) were supplied by Evonik Industries AG (Hanau-Wolfgang, Germany). The purity of all crystalline AA was 99%, with the exception of L-Lysine-HCl (78%)
  6. fHigh- and low-protein diets were supplemented with or without 0.04% (as-fed) of Bacillus spp. product at the expense of cornstarch
  7. g SID standardized ileal digestibility