Effects of Saccharomyces cerevisiae fermentation products on performance and rumen fermentation and microbiota in dairy cows fed a diet containing low quality forage

Table 2 Primers used in this study

Target		Primer sequences (5′→3′)	Product size, bp	Reference
Total bacteria	F	CGGCAACGAGCGCAACCC	130	[27]
Total bacteria	R	CCATTGTAGCACGTGTGTAGCC		[27]
Protozoa	F	GCTTTCGWTGGTAGTGTATT	223	[28]
Protozoa	R	CTTGCCCTCYAATCGTWCT		[28]
Fungi	F	GAGGAAGTAAAAGTCGTAACAAGGTTTC	120	[27]
Fungi	R	CAAATTCACAAAGGGTAGGATGATT		[27]
Ruminococcus albus	F	CCCTAAAAGCAGTCTTAGTTCG	175	[29]
Ruminococcus albus	R	CCTCCTTGCGGTTAGAACA		[29]
R. flavefaciens	F	CGAACGGAGATAATTTGAGTTTACTTAGG	132	[27]
R. flavefaciens	R	CGGTCTCTGTATGTTATGAGGTATTACC		[27]
Fibrobacter succinogenes	F	GTTCGGAATTACTGGGCGTAAA	121	[27]
Fibrobacter succinogenes	R	CGCCTGCCCCTGAACTATC		[27]
Selenomonas ruminantium	F	TGCTAATACCGAATGTTG	515	[30]
Selenomonas ruminantium	R	TCCTGCACTCAAGAAAGA		[30]
Megasphaera elsdenii	F	GACCGAAACTGCGATGCTAGA	128	[31]
Megasphaera elsdenii	R	CGCCTCAGCGTCAGTTGTC		[31]
Streptococcus bovis	F	ATGTTAGATGCTTGAAAGGAGCAA	90	[32]
	R	CGCCTTGGTGAGCCGTTA		[32]

ISSN: 2049-1891