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Table 3 Effects of supplementing cows with inorganic (INO; n = 11) or complexed (AAC; n = 9) trace minerals during the peripartal period on mRNA expression (log-2 scale) of genes related with inflammation response, oxidative stress, eicosanoids, transcription factors and antimicrobial peptides in endometrium tissue at +14 and +30 d after parturition

From: Peripheral leukocyte and endometrium molecular biomarkers of inflammation and oxidative stress are altered in peripartal dairy cows supplemented with Zn, Mn, and Cu from amino acid complexes and Co from Co glucoheptonate

Gene Treatments SEMa P value1
INO AAC
+14 +30 +14 +30 Treatment Time T × Tb
Inflammation
IL10 4.13 3.83 5.13 3.72 0.19 0.04 <0.01 <0.01
IL1B 0.65 -0.37 3.51 -0.98 0.84 0.26 <0.01 <0.01
IL6 2.12 2.91 2.88 2.70 0.23 0.24 0.15 0.03
IL8 1.26 -0.01 4.51 -1.52 0.84 0.37 <0.01 <0.01
MYD88 4.98 4.86 5.46 4.93 0.15 0.06 0.05 0.20
NFKB1 5.06 5.36 5.41 5.06 0.16 0.87 0.90 0.06
SAA3 3.10 2.77 4.88 2.06 1.00 0.46 0.15 0.25
STAT3 5.05 5.22 5.69 5.16 0.19 0.05 0.40 0.11
TLR2 5.05 4.58 5.87 4.39 0.28 0.19 <0.01 0.10
TLR4 5.24 4.49 5.69 3.34 0.18 0.40 <0.01 0.08
TNF 4.21 4.60 5.67 4.32 0.36 0.11 0.12 0.01
Oxidative stress
NFE2L2 4.85 5.11 5.20 5.26 0.13 0.06 0.22 0.43
NOS3 4.94 5.16 5.11 5.07 0.36 0.91 0.76 0.66
NRROS 5.18 4.62 5.20 4.87 0.32 0.68 0.13 0.70
SOD1 5.19 5.04 5.26 5.28 0.09 0.09 0.39 0.30
SOD2 4.83 4.13 5.57 3.83 0.32 0.44 <0.01 0.12
SOD3 4.87 5.27 5.08 5.16 0.26 0.85 0.28 0.45
Eicosanoid synthesis
ALOX5 4.89 4.83 5.08 5.14 0.41 0.51 0.99 0.88
ALOX5AP 4.86 4.92 5.33 4.95 0.38 0.51 0.60 0.48
LTA4H 5.07 5.30 5.24 5.23 0.10 0.51 0.30 0.24
LTC4S 3.91 3.87 4.35 3.98 0.42 0.45 0.62 0.70
PLA2G4A 4.92 5.39 5.44 4.96 0.29 0.86 0.99 0.13
PTGDS 5.26 4.94 5.05 4.87 0.29 0.63 0.34 0.77
PTGES 4.50 3.68 5.53 3.92 0.34 0.10 <0.01 0.15
PTGS2 3.17 4.32 4.94 2.85 0.77 0.83 0.52 0.04
Transcription regulation
PPARA 5.09 5.48 5.20 5.68 0.21 0.48 0.02 0.79
PPARD 5.47 5.13 5.46 5.07 0.15 0.81 0.01 0.89
PPARG 4.72 5.17 4.64 6.03 0.31 0.21 <0.01 0.11
RXRA 5.28 5.24 5.24 5.09 0.13 0.49 0.38 0.60
Antimicrobial peptides
MUC1 2.84 3.78 4.29 4.05 0.46 0.04 0.49 0.25
  1. 1 P values represents the probability of statistical significance for the fixed effects (treatment, time, treatment × time). Statistical differences were declared significant at P ≤ 0.05 and tendencies at P ≤ 0.10
  2. aLargest standard error of the mean is shown
  3. bInteraction of treatment × time