Journal of Animal Science and Biotechnology

Table 3 Log₂ back-transformed LSM of interactions between treatment (TRT) and incubation time (T) on gene transcription, SEM and P values for TRT × T

From: Innate immune responses induced by lipopolysaccharide and lipoteichoic acid in primary goat mammary epithelial cells

		LSM TRT^d × T				SEM	P value
Gene	T	Control	LPS	LTA	L + L	TRT × T	TRT × T
Cytokines
CCL2	3 h	0.45^c	1.83^a,y	0.48^c,z	0.69^b,z	0.11	0.0040
CCL2	6 h	0.53^c	1.41^a,z	0.61^c,y	0.94^b,y	0.11	0.0040
CCL5	3 h	1.40^c,z	1.45^bc,z	1.62^b	2.00^a	0.07	0.0018
CCL5	6 h	1.91^y	1.72^y	1.68	1.83	0.07	0.0018
CXCL6	3 h	0.43^c	1.67^a	0.51^c,y	0.99^b	0.12	0.0274
CXCL6	6 h	0.44^c	1.49^a	0.32^d,z	0.93^b	0.12	0.0274
CXCL8	3 h	0.49^c	2.17^a,y	0.62^c,y	1.34^b,y	0.17	0.0085
CXCL8	6 h	0.52^b	1.24^a,z	0.30^c,z	0.70^b,z	0.17	0.0085
IL6	3 h	1.01^b	3.01^a	1.15^b	2.17^a	0.28	0.1423
IL6	6 h	1.57	2.33	1.41	1.66	0.28	0.1423
Regulatory genes
IFIT3	3 h	0.85^b,z	0.96^a,z	0.96^a,z	1.05^a,z	0.05	0.5151
IFIT3	6 h	1.19^b,y	1.34^a,y	1.48^a,y	1.47^a,y	0.05	0.5151
IRF3	3 h	0.94^b,z	1.01^z	0.99^z	1.12^a	0.07	0.3942
IRF3	6 h	1.18^y	1.18^y	1.28^y	1.26	0.07	0.3942
MYD88	3 h	1.64^b,z	1.95^a,z	1.67^b,z	2.02^a	0.05	0.7335
MYD88	6 h	1.82^b,y	2.16^a,y	1.96^b,y	2.20^a	0.05	0.7335
NFKB1	3 h	0.93^b,z	1.18^a,z	1.12^a,z	1.21^a,z	0.06	0.5318
NFKB1	6 h	1.37^c,y	1.88^a,y	1.62^b,y	1.90^a,y	0.06	0.5318
PTGS2	3 h	0.85^b,z	1.12^a,z	1.07^a,z	1.12^a,z	0.07	0.2535
PTGS2	6 h	1.31^b,y	1.50^y	1.63^a,y	1.48^y	0.07	0.2535
TLR2	3 h	9.46	10.76	10.80	11.41	0.12	0.2028
TLR2	6 h	11.41	12.04	9.47	11.07	0.12	0.2028
TLR4	3 h	0.99^c	1.42^a	1.28^ab,y	1.08^bc,z	0.10	<0.0001
TLR4	6 h	1.09^b	1.57^a	0.85^c,z	1.37^a,y	0.10	<0.0001
TOLLIP	3 h	0.96^b	0.97^b	1.03^a	0.97^b	0.03	0.3689
TOLLIP	6 h	0.96^b	0.94^b	1.06^a	0.94^b	0.03	0.3689

^a-cDifferent letters represent significant differences between treatments within the same incubation time (P < 0.05). The letter a indicates higher transcript levels than b and c. The letter b indicates higher transcript levels than c
^dTreatments: LPS = incubation with 1 μg/mL lipopolysaccharide; LTA = incubation with 20 μg/mL lipoteichoic acid; L + L = incubation with the combination of both toxins; Control = incubation without toxins
^y-zDifferent letters represent significant differences between time points within the same treatment (P < 0.05). The letter y indicates higher transcript levels than z

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