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Table 3 Log2 back-transformed LSM of interactions between treatment (TRT) and incubation time (T) on gene transcription, SEM and P values for TRT × T

From: Innate immune responses induced by lipopolysaccharide and lipoteichoic acid in primary goat mammary epithelial cells

  LSM TRTd × T SEM P value
Gene T Control LPS LTA L + L TRT × T TRT × T
Cytokines
  CCL2 3 h 0.45c 1.83a,y 0.48c,z 0.69b,z 0.11 0.0040
6 h 0.53c 1.41a,z 0.61c,y 0.94b,y
  CCL5 3 h 1.40c,z 1.45bc,z 1.62b 2.00a 0.07 0.0018
6 h 1.91y 1.72y 1.68 1.83
  CXCL6 3 h 0.43c 1.67a 0.51c,y 0.99b 0.12 0.0274
6 h 0.44c 1.49a 0.32d,z 0.93b
  CXCL8 3 h 0.49c 2.17a,y 0.62c,y 1.34b,y 0.17 0.0085
6 h 0.52b 1.24a,z 0.30c,z 0.70b,z
  IL6 3 h 1.01b 3.01a 1.15b 2.17a 0.28 0.1423
6 h 1.57 2.33 1.41 1.66
Regulatory genes
  IFIT3 3 h 0.85b,z 0.96a,z 0.96a,z 1.05a,z 0.05 0.5151
6 h 1.19b,y 1.34a,y 1.48a,y 1.47a,y
  IRF3 3 h 0.94b,z 1.01z 0.99z 1.12a 0.07 0.3942
6 h 1.18y 1.18y 1.28y 1.26
  MYD88 3 h 1.64b,z 1.95a,z 1.67b,z 2.02a 0.05 0.7335
6 h 1.82b,y 2.16a,y 1.96b,y 2.20a
  NFKB1 3 h 0.93b,z 1.18a,z 1.12a,z 1.21a,z 0.06 0.5318
6 h 1.37c,y 1.88a,y 1.62b,y 1.90a,y
  PTGS2 3 h 0.85b,z 1.12a,z 1.07a,z 1.12a,z 0.07 0.2535
6 h 1.31b,y 1.50y 1.63a,y 1.48y
  TLR2 3 h 9.46 10.76 10.80 11.41 0.12 0.2028
6 h 11.41 12.04 9.47 11.07
  TLR4 3 h 0.99c 1.42a 1.28ab,y 1.08bc,z 0.10 <0.0001
6 h 1.09b 1.57a 0.85c,z 1.37a,y
  TOLLIP 3 h 0.96b 0.97b 1.03a 0.97b 0.03 0.3689
6 h 0.96b 0.94b 1.06a 0.94b
  1. a-cDifferent letters represent significant differences between treatments within the same incubation time (P < 0.05). The letter a indicates higher transcript levels than b and c. The letter b indicates higher transcript levels than c
  2. dTreatments: LPS = incubation with 1 μg/mL lipopolysaccharide; LTA = incubation with 20 μg/mL lipoteichoic acid; L + L = incubation with the combination of both toxins; Control = incubation without toxins
  3. y-zDifferent letters represent significant differences between time points within the same treatment (P < 0.05). The letter y indicates higher transcript levels than z
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891