|
LSM TRTd × T
|
SEM
|
P value
|
---|
Gene
|
T
|
Control
|
LPS
|
LTA
|
L + L
|
TRT × T
|
TRT × T
|
---|
Cytokines
|
CCL2
|
3 h
|
0.45c
|
1.83a,y
|
0.48c,z
|
0.69b,z
|
0.11
|
0.0040
|
6 h
|
0.53c
|
1.41a,z
|
0.61c,y
|
0.94b,y
|
CCL5
|
3 h
|
1.40c,z
|
1.45bc,z
|
1.62b
|
2.00a
|
0.07
|
0.0018
|
6 h
|
1.91y
|
1.72y
|
1.68
|
1.83
|
CXCL6
|
3 h
|
0.43c
|
1.67a
|
0.51c,y
|
0.99b
|
0.12
|
0.0274
|
6 h
|
0.44c
|
1.49a
|
0.32d,z
|
0.93b
|
CXCL8
|
3 h
|
0.49c
|
2.17a,y
|
0.62c,y
|
1.34b,y
|
0.17
|
0.0085
|
6 h
|
0.52b
|
1.24a,z
|
0.30c,z
|
0.70b,z
|
IL6
|
3 h
|
1.01b
|
3.01a
|
1.15b
|
2.17a
|
0.28
|
0.1423
|
6 h
|
1.57
|
2.33
|
1.41
|
1.66
|
Regulatory genes
|
IFIT3
|
3 h
|
0.85b,z
|
0.96a,z
|
0.96a,z
|
1.05a,z
|
0.05
|
0.5151
|
6 h
|
1.19b,y
|
1.34a,y
|
1.48a,y
|
1.47a,y
|
IRF3
|
3 h
|
0.94b,z
|
1.01z
|
0.99z
|
1.12a
|
0.07
|
0.3942
|
6 h
|
1.18y
|
1.18y
|
1.28y
|
1.26
|
MYD88
|
3 h
|
1.64b,z
|
1.95a,z
|
1.67b,z
|
2.02a
|
0.05
|
0.7335
|
6 h
|
1.82b,y
|
2.16a,y
|
1.96b,y
|
2.20a
|
NFKB1
|
3 h
|
0.93b,z
|
1.18a,z
|
1.12a,z
|
1.21a,z
|
0.06
|
0.5318
|
6 h
|
1.37c,y
|
1.88a,y
|
1.62b,y
|
1.90a,y
|
PTGS2
|
3 h
|
0.85b,z
|
1.12a,z
|
1.07a,z
|
1.12a,z
|
0.07
|
0.2535
|
6 h
|
1.31b,y
|
1.50y
|
1.63a,y
|
1.48y
|
TLR2
|
3 h
|
9.46
|
10.76
|
10.80
|
11.41
|
0.12
|
0.2028
|
6 h
|
11.41
|
12.04
|
9.47
|
11.07
|
TLR4
|
3 h
|
0.99c
|
1.42a
|
1.28ab,y
|
1.08bc,z
|
0.10
|
<0.0001
|
6 h
|
1.09b
|
1.57a
|
0.85c,z
|
1.37a,y
|
TOLLIP
|
3 h
|
0.96b
|
0.97b
|
1.03a
|
0.97b
|
0.03
|
0.3689
|
6 h
|
0.96b
|
0.94b
|
1.06a
|
0.94b
|
-
a-cDifferent letters represent significant differences between treatments within the same incubation time (P < 0.05). The letter a indicates higher transcript levels than b and c. The letter b indicates higher transcript levels than c
-
dTreatments: LPS = incubation with 1 μg/mL lipopolysaccharide; LTA = incubation with 20 μg/mL lipoteichoic acid; L + L = incubation with the combination of both toxins; Control = incubation without toxins
-
y-zDifferent letters represent significant differences between time points within the same treatment (P < 0.05). The letter y indicates higher transcript levels than z