Innate immune responses induced by lipopolysaccharide and lipoteichoic acid in primary goat mammary epithelial cells

Table 1 Quantitative PCR performance of the measured genes

Gene	Median Ct^a	Median ∆Ct^b	Slope^c	(R²)^d	Efficiency^e
CCL2	28.62	9.66	−3.29	0.997	2.011
CCL5	28.95	10.05	−3.28	0.991	2.019
CXCL6	24.29	5.23	−3.19	0.999	2.060
CXCL8	29.26	10.34	−3.11	0.994	2.097
IFIT3	24.96	6.04	−3.07	0.993	2.117
IL6	29.11	10.12	−3.34	0.993	1.992
IRF3	24.16	5.27	−3.09	0.991	2.108
MYD88	24.62	5.71	−3.02	0.991	2.143
NFKB1	26.58	7.62	−2.91	0.996	2.204
PTGS2	27.47	8.49	−3.06	0.986	2.120
TLR2	28.51	9.64	−3.31	0.999	2.006
TLR4	30.20	11.28	−2.94	0.999	2.189
TOLLIP	23.59	4.75	−3.35	0.995	1.989

^aThe median is calculated considering all time points and treatments
^bThe median of ∆Ct is calculated as [Ct gene - geometrical mean of Ct internal controls] for each time point and treatment
^cSlope of the standard curve
^dR² stands for the coefficient of determination of the standard curve
^eEfficiency is calculated as [10^(−1/Slope)]