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Table 1 Quantitative PCR performance of the measured genes

From: Innate immune responses induced by lipopolysaccharide and lipoteichoic acid in primary goat mammary epithelial cells

Gene Median Cta Median ∆Ctb Slopec (R2)d Efficiencye
CCL2 28.62 9.66 −3.29 0.997 2.011
CCL5 28.95 10.05 −3.28 0.991 2.019
CXCL6 24.29 5.23 −3.19 0.999 2.060
CXCL8 29.26 10.34 −3.11 0.994 2.097
IFIT3 24.96 6.04 −3.07 0.993 2.117
IL6 29.11 10.12 −3.34 0.993 1.992
IRF3 24.16 5.27 −3.09 0.991 2.108
MYD88 24.62 5.71 −3.02 0.991 2.143
NFKB1 26.58 7.62 −2.91 0.996 2.204
PTGS2 27.47 8.49 −3.06 0.986 2.120
TLR2 28.51 9.64 −3.31 0.999 2.006
TLR4 30.20 11.28 −2.94 0.999 2.189
TOLLIP 23.59 4.75 −3.35 0.995 1.989
  1. aThe median is calculated considering all time points and treatments
  2. bThe median of ∆Ct is calculated as [Ct gene - geometrical mean of Ct internal controls] for each time point and treatment
  3. cSlope of the standard curve
  4. dR2 stands for the coefficient of determination of the standard curve
  5. eEfficiency is calculated as [10(−1/Slope)]