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Table 1 Effect of time of incubation of sperm at 37 °C on sperm DNA fragmentation (sDF, %) within each centrifugation procedure (UDC, SW and SLC)

From: New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models

  T0 T3 T6 T24 P-value
UDC 12.98 ± 1.52c 16.47 ± 1.40c 20.90 ± 1.34b 35.86 ± 3.21a <0.001
SW 13.35 ± 1.17c 15.01 ± 1.27bc 18.28 ± 2.21b 34.84 ± 2.60a <0.001
SLC 11.48 ± 1.67b 14.02 ± 2.16b 15.70 ± 2.26b 24.91 ± 2.19a <0.001
  1. T0, T3, T6, T24 incubation for 0, 3, 6, 24 h at 37 °C, UDC uncentrifuged diluted control, SW sperm washing, SLC single layer centrifugation
  2. Values are expressed as mean ± standard error of the mean (SEM)
  3. Different letters indicate significant differences