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Table 1 Effect of time of incubation of sperm at 37 °C on sperm DNA fragmentation (sDF, %) within each centrifugation procedure (UDC, SW and SLC)

From: New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models

 

T0

T3

T6

T24

P-value

UDC

12.98 ± 1.52c

16.47 ± 1.40c

20.90 ± 1.34b

35.86 ± 3.21a

<0.001

SW

13.35 ± 1.17c

15.01 ± 1.27bc

18.28 ± 2.21b

34.84 ± 2.60a

<0.001

SLC

11.48 ± 1.67b

14.02 ± 2.16b

15.70 ± 2.26b

24.91 ± 2.19a

<0.001

  1. T0, T3, T6, T24 incubation for 0, 3, 6, 24 h at 37 °C, UDC uncentrifuged diluted control, SW sperm washing, SLC single layer centrifugation
  2. Values are expressed as mean ± standard error of the mean (SEM)
  3. Different letters indicate significant differences
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891