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Table 2 Antihypertensive peptides generated from the hydrolysis of animal products

From: Protein hydrolysates in animal nutrition: Industrial production, bioactive peptides, and functional significance

Source Protease(s) Amino acid sequence IC50, μmol/La
Pig muscle myosin Thermolysin Ile-Thr-Thr-Asn-Pro 549
Pig muscle myosin Pepsin Lys-Arg-Val-Ile-Thr-Tyr 6.1
Pig muscle actin Pepsin Val-Lys-Arg-Gly-Phe 20.3
Pig muscle troponin Pepsin Lys-Arg-Gln-Lys-Tyr-Asp-Ile 26.2
Pig muscle Pepsin + Pancreatin Lys-Leu-Pro 500
Pig muscle Pepsin + Pancreatin Arg-Pro-Arg 382
Chicken muscle Thermolysin Leu-Ala-Pro 3.2
Chicken muscle myosin Thermolysin Phe-Gln-Lys-Pro-Lys-Arg 14
Chicken muscle Thermolysin Ile-Lys-Trp 0.21
Chicken collagen Aspergillus proteases + Proteases FP, A, G and N Gly-Ala-X-Gly-Leu-X-Gly-Pro 29.4
Cow muscle Thermolysin + Proteinase A Val-Leu-Ala-Gln-Tyr-Lys 32.1
Cow muscle Thermolysin + Proteinase A Phe-His-Gly 52.9
Cow muscle Proteinase K Gly-Phe-His-Ile 64.3
Cow skin gelatin Alcalase + Pronase E + Collagenase Gly-Pro-Val 4.67
Cow skin gelatin Alcalase + Pronase E + Collagenase Gly-Pro-Leu 2.55
Bonito (fish) muscle Thermolysin Leu-Lys-Pro-Asn-Met 2.4
Bonito (fish) muscle Thermolysin Leu-Lys-Pro 0.32
Bonito (fish) muscle Thermolysin Ile-Lys-Pro 6.9
Salmon muscle Thermolysin Val-Trp 2.5
Salmon muscle Thermolysin Met-Trp 9.9
Salmon muscle Thermolysin Ile-Trp 4.7
Sardine muscle Alcalase Ile-Tyr 10.5
Sardine muscle Alcalase Ala-Lys-Lys 3.13
Sardine muscle Alcalase Gly-Trp-Ala-Pro 3.86
Sardine muscle Alcalase Lys-Tyr 1.63
Alaska pollack skin Alcalase + Pronase + Collagenase Gly-Pro-Leu 2.65
Alaska pollack skin Alcalase + Pronase + Collagenase Gly-Pro-Met 17.1
Shark muscle Protease SM98011 Glu-Tyr 1.98
Shark muscle Protease SM98012 Phe-Glu 2.68
Shark muscle Protease SM98013 Cys-Phe 1.45
Egg yolk Pepsin Tyr-Ile-Glu-Ala-Val-Asn-Lys-Val-Ser-Pro-Arg-Ala-Gly-Gln-Phe 9.4b
Egg yolk Pepsin Tyr-Ile-Asn-Gln-Met-Pro-Gln-Lys-Ser-Arg-Glu 10.1b
  1. Adapted from Ryan JT et al. [28], Ryder et al. [33], and Zambrowicz et al. [34]
  2. “X” hydroxyproline
  3. aInhibition of angiotensin-I converting enzyme (ACE) activity. All values are expressed as μM, except for egg yolk-derived peptides (μg/mL) as indicated by a superscript “b”