Class of enzyme | Name of enzyme | EC number | Specific cleavage |
---|---|---|---|
Endopeptidases | |||
 Aspartate protease | Chymosin (rennin; pH 1.8–2) | 3.4.23.4 | the Phe-Met bond, clotting of milk |
Pepsin A (pH 1.8–2) | 3.4.23.1 | Aromatic AAs, hydrophobic AAs | |
 Cysteine protease | Bromelain (from pieapples) | 3.4.22.4 | Ala, Gly, Lys, Phe, Tyr |
Cathepsin B | 3.4.22.1 | Arg, Lys, Phe | |
Ficain (ficin; from fig tree) | 3.4.22.3 | Ala, Asn, Gly, Leu, Lys, Tyr, Val | |
Papain (from papaya) | 3.4.22.2 | Arg, Lys, Phe | |
 Metallo protease | Bacillolysin (Bacillus bacteria) | 3.4.24.28 | Aromatic AAs, Ile, Leu, Val |
Thermolysin (Bacillus bacteria) | 3.4.24.27 | Aromatic AAs, Ile, Leu, Val | |
 Serine protease | Chymotrypsin (pH 8–9) | 3.4.21.1 | Aromatic AAs, Leu |
Subtilisin (from Bacillus bacteria) | 3.4.21.14 | Mainly hydrophobic AAs | |
Trypsin (pH 8–9) | 3.4.21.4 | Arg, Lys | |
Exopeptidases | |||
 Aminopeptidases | Aminopeptidasea | 3.4.11.1 | AA at the N-terminus of protein/peptide |
Aminopeptidase Yb | 3.4.11.15 | Lys at the N-terminus of protein/peptide | |
 Carboxypeptidase | Carboxypeptidasec | 3.4.16.1 | Acidic, neutral, and basic AAs |
Glycine carboxypeptidased | 3.4.17.4 | Gly at the C-terminus of protein/peptide | |
Alanine carboxypeptidasee | 3.4.17.8 | D-Ala at the C-terminus of peptide | |
Carboxypeptidase Sf | 3.4.17.9 | Gly at the C-terminus of protein/peptide | |
 Dipeptidase | Dipeptidase 1f | 3.4.13.11 | A wide range of dipeptides |
Proline dipeptidase (prolidase)a | 3.4.13.9 | AA-Pro or -hydroxyproline at the C-terminus (not Pro-Pro) | |
Prolyl dipeptidasea | 3.3.13.8 | Pro-AA or Hydroxyproline-AA | |
Endo- and exo-peptidases | |||
 Pronase | A mixture of proteasesa (from Streptomyces griseus) | 3.4.24 | Acidic, neutral, and basic AAs |
 Other peptidases | Dipeptidyl-peptide IIIf | 3.4.14.4 | Release of an N-terminal dipeptide from a peptide comprising four or more AA residues, with broad specificity |
Dipeptidyl-peptidase IVg | 3.4.14.5 | Release of an N-terminal dipeptide from a peptide consisting of prolineh |