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Table 5 Log2-backtransformed least squares means of liver and polymorphonuclear leukocyte (PMNL) gene expression data in transition cows supplemented with rumen-protected methionine (MET) or unsupplemented (CON) at -10, +7, and +30 d from calving

From: Expression of fatty acid sensing G-protein coupled receptors in peripartal Holstein cows

       Diet × Time     
Gene Diet Time MET CON SEM1 P-value
  MET CON −10 +7 +30 −10 +7 +30 −10 +7 +30   Diet Time Diet × Time
Liver
GPR40 Not expressed
GPR84 0.77 0.84 0.88 0.74 0.80 0.85 0.73 0.75 0.91 0.75 0.86 0.14 0.64 0.25 0.89
GPR120 Not expressed
GPR109A 0.51 0.53 0.64 0.48 0.46 0.57 0.52 0.44 0.72 0.44 0.47 0.15 0.77 0.22 0.61
PMNL
GPR40 0.85* 1.13* 1.15a 0.95a,b 0.85b 1.00 0.80 0.77 1.33 1.14 0.94 0.17 0.04 0.04 0.78
GPR84 1.49* 0.65* 0.96 1.05 0.96 1.22 1.82 1.49 0.75 0.60 0.61 0.64 0.04 0.93 0.51
GPR120 Not expressed
GPR109A 1.34 0.87 1.26 0.91 1.10 1.42 1.31 1.28 1.11 0.63 0.94 0.44 0.09 0.60 0.71
  1. a,bStatistical difference (P < 0.05) among time points within the same group
  2. *Statistical difference (P < 0.05) between groups within time points
  3. 1Largest standard error of the mean