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Table 5 Log2-backtransformed least squares means of liver and polymorphonuclear leukocyte (PMNL) gene expression data in transition cows supplemented with rumen-protected methionine (MET) or unsupplemented (CON) at -10, +7, and +30 d from calving

From: Expression of fatty acid sensing G-protein coupled receptors in peripartal Holstein cows

      

Diet × Time

    

Gene

Diet

Time

MET

CON

SEM1

P-value

 

MET

CON

−10

+7

+30

−10

+7

+30

−10

+7

+30

 

Diet

Time

Diet × Time

Liver

GPR40

Not expressed

GPR84

0.77

0.84

0.88

0.74

0.80

0.85

0.73

0.75

0.91

0.75

0.86

0.14

0.64

0.25

0.89

GPR120

Not expressed

GPR109A

0.51

0.53

0.64

0.48

0.46

0.57

0.52

0.44

0.72

0.44

0.47

0.15

0.77

0.22

0.61

PMNL

GPR40

0.85*

1.13*

1.15a

0.95a,b

0.85b

1.00

0.80

0.77

1.33

1.14

0.94

0.17

0.04

0.04

0.78

GPR84

1.49*

0.65*

0.96

1.05

0.96

1.22

1.82

1.49

0.75

0.60

0.61

0.64

0.04

0.93

0.51

GPR120

Not expressed

GPR109A

1.34

0.87

1.26

0.91

1.10

1.42

1.31

1.28

1.11

0.63

0.94

0.44

0.09

0.60

0.71

  1. a,bStatistical difference (P < 0.05) among time points within the same group
  2. *Statistical difference (P < 0.05) between groups within time points
  3. 1Largest standard error of the mean