Novel SNPs in IL-17F and IL-17A genes associated with somatic cell count in Chinese Holstein and Inner-Mongolia Sanhe cattle

Table 1 Primer pairs of PCR and real time qRT-PCR used in the present study

SNP	Gene	Upper Primer (5’–3’)	Lower Primer (5’–3’)	PCR type
1	IL-17F	GTCATTGGAACATCTCAGGAC	GCAGATCAGCTCAGCTGAAGA	Normal PCR
2	IL-17F	ATAATAGTCCTTACATTGACT	GCAAAGCATCAGGAGAGGTTG	Normal PCR
3	IL-17F	TGACCTGCTTACTGCCTAAGT	TAAGAGATTTGCTATAGAGTG	Normal PCR
4	IL-17F	TGACCTGCTTACTGCCTAAGT	TAAGAGATTTGCTATAGAGTG	Normal PCR
5	IL-17F	AACCAAAATGGATTAGCAAGT	GTACAGGGCCAGTTAGTGAA	Normal PCR
6	IL-17F	AACCAAAATGGATTAGCAAGT	GTACAGGGCCAGTTAGTGAA	Normal PCR
7	IL-17F	AACCAAAATGGATTAGCAAGT	GTACAGGGCCAGTTAGTGAA	Normal PCR
8	IL-17F	AACCAAAATGGATTAGCAAGT	GTACAGGGCCAGTTAGTGAA	Normal PCR
9	IL-17A	CAGTTCAAGTACACAAATGAGC	GGTGTTTATCCATCCTACATAC	Normal PCR
10	IL-17A	TATGAGTATCTGTTTTGCCTAG	CAGTTAGACTTGCTGTCTCTCT	Normal PCR
11	IL-17A	TATGAGTATCTGTTTTGCCTAG	CAGTTAGACTTGCTGTCTCTCT	Normal PCR
12	IL-17A	TATGAGTATCTGTTTTGCCTAG	CAGTTAGACTTGCTGTCTCTCT	Normal PCR
	IL-17A	AGGGTCAACCTAAACATCGTT	GTACCTCTCAGGGTCCTCATT	Real-time PCR
	IL-4	AGGGTTGGAATTGAGCTTAGG	TGGCTTCATTCACAGAACAGG	Real-time PCR
	GADPH	GCTGCTTTTAATTCTGGC	CTTTCCATTGATGACGAG	Real-time PCR

ISSN: 2049-1891