Transfer of β-hydroxy-β-methylbutyrate from sows to their offspring and its impact on muscle fiber type transformation and performance in pigs

Table 3 The primer sequences of the target genes

Target gene	Primer sequence (5′to 3′)	Product size, bp	GenBank No.
MyHC1	F: GTTTGCCAACTATGCTGGGG	95	NM_213855.1
MyHC1	R: TGTGCAGAGCTGACACAGTC	95	NM_213855.1
MyHCIIa	F: CTCTGAGTTCAGCAGCCATGA	127	NM_214136.1
MyHCIIa	R: GATGTCTTGGCATCAAAGGGC	127	NM_214136.1
MyHCIIx	F: TTGACTGGGCTGCCATCAAT	111	NM_001104951.1
MyHCIIx	R: GCCTCAATGCGCTCCTTTTC	111	NM_001104951.1
MyHCIIb	F: GAGGTACATCTAGTGCCCT	83	NM_001123141.1
MyHCIIb	R: GCAGCCTCCCCAAAAATAGC	83	NM_001123141.1
Pax7	F: TGAAGGTCGGAGTGAACGGAT	74	NM_001206359.1
Pax7	R: CACTTTGCCAGAGTTAAAAGCA	74	NM_001206359.1
IGF-1	F: CACAGACGGGCATCGTGGAT	90	NM_214256.1
IGF-1	R: ACTTGGCAGGCTTGAGGGGT	90	NM_214256.1
mTOR	F: CATTGGAGATGGTTTGGTGA	160	XM_003127584.5
mTOR	R: ATGGGATGTGGCTTGTTTGA	160	XM_003127584.5
MRF4	F: CCTTCGGTGCCTTTCTTCCAT	88	NM_001244672.1
MRF4	R: GAGTTATTTCTCCCCCACTTCC	88	NM_001244672.1
MSTN	F: TTTACCTGTTTATGCTGATTGTTG	194	NM_214435.2
MSTN	R: TTTGCTAATGTTAGGAGCTGTTTC	194	NM_214435.2
FoxO1	F: TCAAGGATAAGGGCGACAGC	95	NM_214014.2
FoxO1	R: AATGTCATTATGGGGAGGAGAGT	95	NM_214014.2
Sox6	F: CGGATTGGGGAGTATAAGCA	159	XM_01399454.1
Sox6	R: CATCTGAGGTGATGGTGTGG	159	XM_01399454.1
MAFbx	F: CCCTCTCATTCTGTCACCTTG	104	NM_001044588
MAFbx	R: ATGTGCTCTCCCACCATAGC	104	NM_001044588
MuRF1	F: GCTGGATTGGAAGAAGATGTAT	144	NW_001184756
MuRF1	R: AGGAAAGAATGTGGCAGTGTCT	144	NW_001184756
GAPDH	F: TGAAGGTCGGAGTGAACGGAT	74	NM_001206359.1
GAPDH	R: CACTTTGCCAGAGTTAAAAGCA	74	NM_001206359.1

MyHC myosin heavy chain, Pax7 paired box 7, IGF-I insulin-like growth factor-I, mTOR mammalian target of rapamycin, MRF4 muscle regulator factor 4, MSTN myostatin, FoxO1 forkhead box transcription factor O1, MAFbx muscle atrophy F-box, MuRF1 muscle Ring finger 1, GAPDH glyceraldehyde-3-phosphate dehydrogenase

ISSN: 2049-1891