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Table 1 Differentially expressed (>3-fold) proteins of spermatozoa at different steps of cryopreservation

From: Proteomic identification of cryostress in epididymal spermatozoa

Spot no. Protein NCBI no. MASCOT scorea Expression ratiob Search
228 Major fibrous sheath protein chain D, bovine mitochondrial gi|4588120 88.0 3.87 NCBI
1304 F1-ATPase complexed with aurovertin B gi|1827812 183.0 12.57 NCBI
2311 PREDICTED: Uncharacterized protein LOC616410 gi|119914715 299.0 0.25 NCBI
4208 Triosephosphate isomerase gi|61888856 174.0 6.48 NCBI
4418 Nucleoside diphosphate kinase 7 gi|62751773 64.0 6.13 SwissProt
4607 Outer dense fiber protein 2 gi|84000345 290.0 0.46 NCBI
5204 NADH dehydrogenase flavoprotein 2 gi|72004 67.0 5.30 SwissProt
5305 F-actin-capping protein subunit beta gi|28603770 53.0 5.73 NCBI
7206 Superoxide dismutase, mitochondrial gi|88853816 74.0 3.92 NCBI
  1. aMASCOT scores are −10×log(P), where P is the probability that the observed match is a random event. Individual scores > 40 indicate identity or extensive homology (P < 0.05)
  2. bExpression ratio is the ratio of relative volume of protein spots with after cryopreservation value to over before