Proteomic identification of cryostress in epididymal spermatozoa

Table 1 Differentially expressed (>3-fold) proteins of spermatozoa at different steps of cryopreservation

Spot no.	Protein	NCBI no.	MASCOT score^a	Expression ratio^b	Search
228	Major fibrous sheath protein chain D, bovine mitochondrial	gi\|4588120	88.0	3.87	NCBI
1304	F1-ATPase complexed with aurovertin B	gi\|1827812	183.0	12.57	NCBI
2311	PREDICTED: Uncharacterized protein LOC616410	gi\|119914715	299.0	0.25	NCBI
4208	Triosephosphate isomerase	gi\|61888856	174.0	6.48	NCBI
4418	Nucleoside diphosphate kinase 7	gi\|62751773	64.0	6.13	SwissProt
4607	Outer dense fiber protein 2	gi\|84000345	290.0	0.46	NCBI
5204	NADH dehydrogenase flavoprotein 2	gi\|72004	67.0	5.30	SwissProt
5305	F-actin-capping protein subunit beta	gi\|28603770	53.0	5.73	NCBI
7206	Superoxide dismutase, mitochondrial	gi\|88853816	74.0	3.92	NCBI

^aMASCOT scores are −10×log(P), where P is the probability that the observed match is a random event. Individual scores > 40 indicate identity or extensive homology (P < 0.05)
^bExpression ratio is the ratio of relative volume of protein spots with after cryopreservation value to over before

ISSN: 2049-1891