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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: Proteomic identification of cryostress in epididymal spermatozoa

Fig. 4

2-DE Separation of proteins by 2-DE. 2-DE gels were stained with silver nitrate and analyzed using PDQuest 8.0 software. Protein spots from (a) before cryopreservation and after cryopreservation. b The expression of seven proteins increased significantly after cryopreservation. c The expression of two proteins decreased significantly after cryopreservation. Differentially expressed (>3-fold) proteins were determined by comparing samples before and after cryopreservation (P < 0.05, n = 9). The line indicates the landmark of equal levels for before cryopreservation

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