Fig. 4
From: Proteomic identification of cryostress in epididymal spermatozoa
2-DE Separation of proteins by 2-DE. 2-DE gels were stained with silver nitrate and analyzed using PDQuest 8.0 software. Protein spots from (a) before cryopreservation and after cryopreservation. b The expression of seven proteins increased significantly after cryopreservation. c The expression of two proteins decreased significantly after cryopreservation. Differentially expressed (>3-fold) proteins were determined by comparing samples before and after cryopreservation (P < 0.05, n = 9). The line indicates the landmark of equal levels for before cryopreservation