Table 3 Effect of oocyte activation, semen type and sperm selection technique for ICSI on fertilization percentage
From: Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm
Semen type | Frozen-thawed semen | Fresh | Frozen-thawed | ||
|---|---|---|---|---|---|
Oocyte treatment | Mechanical activation | Activated with 7 % Ethanol | |||
Sperm Treatment | “Swim-up” | “Swim-up” | “Swim-up” + Zona pellucida | “Swim-up” | “Swim-up” + Zona pellucida |
N | 17 | 5 | 6 | 5 | 5 |
n | 200 | 123 | 73 | 70 | 51 |
Activated ooctyes, % | 4,9 25 ± 2.7 | 7,5 61 ± 1.3* | 3,6 49 ± 1.6* | 4,0 57 ± 3.2* | 3,1 61 ± 6.3* |
Fertilized ooyctes, % | 8 4 ± 0.2 | 3,5 28 ± 1.7* | 18 25 ± 1.7* | 9 13 ± 1.2*▲ | 6 12 ± 0.9▲ |