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Table 2 Viability and acrosome status of sperm selected by swim-up or swim-up and oocyte ZP binding

From: Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

  Fresh Semen, %   Frozen-thawed semen, %
AR/A AR/D NAR/A NAR/D AR/A AR/D NAR/A NAR/D
SU N = 12 N = 14
  n = 1,194 52,8
44 ± 1.8a
17,9
15 ± 0.8a
34,0
28 ± 8.3a
14,7
12 ± 0.6a
n = 1410 71,8
51 ± 0.9a*
37,3
26 ± 0.9a*
17,5
12 ± 0.3a*
14,4
10 ± 0.5a
SU + ZP N = 11 N = 19
  n = 1,100 64,8
59 ± 0.9b
34,7
32 ± 1.1b
40
4 ± 0.3b
65
6 ± 0.2b
n = 1350 92,2
68 ± 1.8b*
23,7
18 ± 0.9b*
14,3
11 ± 0.5a*
48
4 ± 0.2b*
  1. SU swim-up, ZP Zona pellucida, AR/A acrosome reaction/alive, AR/D acrosome reaction/dead, NAR/A no acrosome reaction/alive, NAR/D no acrosome reaction/dead, N number of replicates, n number of sperm examined
  2. Percentage data are presented as mean ± SD
  3. a,bValues in the same column with different letters are significantly different (P < 0.05)
  4. *Within rows, among the variables corresponding to fresh and frozen-thawed semen counterparts, significant difference was observed (P < 0.05)