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Table 2 Viability and acrosome status of sperm selected by swim-up or swim-up and oocyte ZP binding

From: Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

 

Fresh Semen, %

 

Frozen-thawed semen, %

AR/A

AR/D

NAR/A

NAR/D

AR/A

AR/D

NAR/A

NAR/D

SU

N = 12

N = 14

 

n = 1,194

52,8

44 ± 1.8a

17,9

15 ± 0.8a

34,0

28 ± 8.3a

14,7

12 ± 0.6a

n = 1410

71,8

51 ± 0.9a*

37,3

26 ± 0.9a*

17,5

12 ± 0.3a*

14,4

10 ± 0.5a

SU + ZP

N = 11

N = 19

 

n = 1,100

64,8

59 ± 0.9b

34,7

32 ± 1.1b

40

4 ± 0.3b

65

6 ± 0.2b

n = 1350

92,2

68 ± 1.8b*

23,7

18 ± 0.9b*

14,3

11 ± 0.5a*

48

4 ± 0.2b*

  1. SU swim-up, ZP Zona pellucida, AR/A acrosome reaction/alive, AR/D acrosome reaction/dead, NAR/A no acrosome reaction/alive, NAR/D no acrosome reaction/dead, N number of replicates, n number of sperm examined
  2. Percentage data are presented as mean ± SD
  3. a,bValues in the same column with different letters are significantly different (P < 0.05)
  4. *Within rows, among the variables corresponding to fresh and frozen-thawed semen counterparts, significant difference was observed (P < 0.05)