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Table 1 Activation procedures in sheep oocytes matured in vitro

From: Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

 

Mechanical activation

Chemical activation

Sham

ICSI

Sham Injection

ETL

CAI

ION

N

13

17

10

20

19

4

n

151

200

78

393

350

78

Activated oocytes,%

2,0

13 ± 1.6a

4,9

25 ± 0.5ab

25

32 ± 3.4b

21,1

54 ± 1.3c

16,4

47 ± 1.3c

3,3

42 ± 5.0c

  1. Sham: oocytes manipulated as in ICSI but no injection; Sham injection: oocyte pierced but no sperm insertion; ETL: 7 % Ethanol 5 min; CAI: 50 μmol/L Calcium Ionophore 10 min; ION: 5 μmol/L Ionomicine 5 min. Mechanically activated oocytes: Oocytes in metaphase II. Activated oocytes: Presence of one pronucleus
  2. N number of replicates; n number of oocytes examined
  3. Percentage data are presented as mean ± SD
  4. a,b,cValues with different letters indicate statistically significant differences (P < 0.05)