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Table 1 Activation procedures in sheep oocytes matured in vitro

From: Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

  Mechanical activation Chemical activation
Sham ICSI Sham Injection ETL CAI ION
N 13 17 10 20 19 4
n 151 200 78 393 350 78
Activated oocytes,% 2,0
13 ± 1.6a
4,9
25 ± 0.5ab
25
32 ± 3.4b
21,1
54 ± 1.3c
16,4
47 ± 1.3c
3,3
42 ± 5.0c
  1. Sham: oocytes manipulated as in ICSI but no injection; Sham injection: oocyte pierced but no sperm insertion; ETL: 7 % Ethanol 5 min; CAI: 50 μmol/L Calcium Ionophore 10 min; ION: 5 μmol/L Ionomicine 5 min. Mechanically activated oocytes: Oocytes in metaphase II. Activated oocytes: Presence of one pronucleus
  2. N number of replicates; n number of oocytes examined
  3. Percentage data are presented as mean ± SD
  4. a,b,cValues with different letters indicate statistically significant differences (P < 0.05)