Table 3 Primer sequences and conditions used for real-time PCR
Product | Sequence | Concentration | ||
|---|---|---|---|---|
Primer | Sequence | size, bp | GenBank ID | nmol/La |
Occludin | ||||
Forward | 5′-GCAGCAGTGGTAACTTGGA-3′ | 113 | NM_001163647.2 | 200 |
Reverse | 5′-GTCGTGTAGTCTGTCTCGTAATG-3′ | |||
ZO-1 | ||||
Forward | 5′-CGGCGAAGGTAATTCAGTGT-3′ | 109 | XM_003353439.2 | 200 |
Reverse | 5′-CGGTTTGGTGGTCTGTAAGT-3′ | |||
Actin β | ||||
Forward | 5′-GCCCGTCCATCGTCCACCG-3′ | 127 | XM_003357928 | 200 |
Reverse | 5′-CAGGAGGCTGGCATGAGGTGTG-3′ | |||