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Table 3 Primer sequences and conditions used for real-time PCR

From: Acetylsalicylic acid supplementation improves protein utilization efficiency while vitamin E supplementation reduces markers of the inflammatory response in weaned pigs challenged with enterotoxigenic E. coli

   Product Sequence Concentration
Primer Sequence size, bp GenBank ID nmol/La
Occludin
 Forward 5′-GCAGCAGTGGTAACTTGGA-3′ 113 NM_001163647.2 200
 Reverse 5′-GTCGTGTAGTCTGTCTCGTAATG-3′    
ZO-1
 Forward 5′-CGGCGAAGGTAATTCAGTGT-3′ 109 XM_003353439.2 200
 Reverse 5′-CGGTTTGGTGGTCTGTAAGT-3′    
Actin β
 Forward 5′-GCCCGTCCATCGTCCACCG-3′ 127 XM_003357928 200
 Reverse 5′-CAGGAGGCTGGCATGAGGTGTG-3′    
  1. aFinal concentration of primers used in real-time PCR