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Table 3 Effect of diet (D) and period (P) on the relative expressiona of key genes in ruminal epithelial tissue

From: Examination of the molecular control of ruminal epithelial function in response to dietary restriction and subsequent compensatory growth in cattle

  RES ADLIB Significance
Geneb Period 1 Period 2 Period 1 Period 2 SEMc D P D*Pd
Growth and Structure
CCND1 5.804 16.219 7.981 11.04 2.701 0.586 0.018 0.182
CCND2 6.544 20.787 13.521 13.724 4.054 0.618 0.183 0.192
CCND3 6.156 50.062 9.498 37.621 9.179 0.957 <0.001 0.396
CCNE1 20.302 43.776 31.736 30.536 8.105 0.913 0.185 0.137
CCNE2 58.975 106.52 86.475 79.928 27.07 0.913 0.948 0.325
CDKN1A 11.429 21.017 13.367 13.944 4.724 0.590 0.288 0.347
CDKN2A 5.6178 14.964 12.357 17.614 3.236 0.245 0.039 0.532
CDKN2B 13.851 23.052 14.281 12.594 5.554 0.372 0.503 0.334
DSG1 7.82 50.432 40.351 27.469 13.02 0.273 0.051 0.019
VFA activation
ACS 2.72 2.359 2.12 2.588 0.155 0.826 0.103 0.733
ACSS1 2.579 2.047 2.703 2.079 0.338 0.818 0.096 0.893
PCCA 1.75 1.911 1.498 1.896 0.128 0.303 0.004 0.358
Transport proteins
CACT 6.597 8.145 10.829 4.969 1.61 0.745 0.189 0.027
NHE1 17.983 7.76 15.672 8.039 1.238 0.418 <0.001 0.302
NHE2 3.455 2.964 2.94 3.197 0.355 0.695 0.745 0.300
NHE3 2.609 8.503 2.759 6.313 0.79 0.212 <0.001 0.148
Ketogenesis
ACAT 12.153 31.857 12.779 20.89 4.802 0.289 0.006 0.235
HMGCL 2.313 5.241 3.169 5.171 0.42 0.356 <0.001 0.278
BDH1 3.971 1.67 3.29 1.99 0.251 0.476 <0.001 0.053
BDH2 3.491 2.803 2.1 2.931 0.304 0.045 0.815 0.017
HMGCS1 6.51 3.476 6.989 2.607 0.779 0.804 <0.001 0.392
HMGCS2 2.641 3.676 2.21 4.586 0.401 0.554 <0.001 0.103
Pyruvate metabolism
LDHa 1.915 1.719 1.609 1.789 0.092 0.207 0.931 0.048
LDHb 1.749 2.151 1.685 2.074 0.121 0.564 0.002 0.959
PC 2.001 2.928 2.409 2.986 0.247 0.355 0.004 0.484
PDHA1 2.074 1.248 1.619 1.265 0.093 0.024 <0.001 0.015
  1. aGene expression values were normalized to the reference gene after adjustment for efficiencies and interplate variation and converted to values relative to the greatest cycle threshold (Ct) within each data set
  2. b CCND1 cyclin D1, CCND2 cyclin D2, CCND3 cyclin D3, CCNE1 cyclin E1, CCNE2 cyclin E2, CDKN1A cyclin dependant kinase inhibitor 1A, CDKN2A cyclin dependant kinase inhibitor 2A, CDKN2B cyclin dependant kinase inhibitor 2B, DSG1 desmoglein 1, ACS acetyl coA synthetase, ACSS1 acyl coA synthetase short-chain family member 1, PCCA propionyl coA carboxylase α polypeptide, CACT solute carrier family 25, carnitine/acylcarnitine translocase member 20, NHE1 NA+/H+ exchanger isoform 1, NHE2 NA+/H+ exchanger isoform 2, NHE3 NA+/H+ exchanger isoform 3, ACAT acetyl coA acyltransferase, HMGCL 3-Hydroxymethyl-3-methylglutaryl-coA lyase, hydroxymethylglutaricaacidura, BDH1 3-Hydroxybutyrate dehydrogenase, type 1, BDH2 3-Hydroxybutyrate dehydrogenase, type 2, HMGCS1 3-Hydroxy-3-methylglutaryl-coA synthase 1, HMGCS2 3-Hydroxy-3-methylglutaryl-coA synthase 2, LDHa lactate dehydrogenase isoform a, LDHb lactate dehydrogenase isoform b, PC pyruvate carboxylase, PDHA1 pyruvate dehydrogenase lipoamide, α 1
  3. cSEM, standard error of the mean
  4. dD*P, diet × period interaction