Skip to main content

Table 3 Effect of diet (D) and period (P) on the relative expressiona of key genes in ruminal epithelial tissue

From: Examination of the molecular control of ruminal epithelial function in response to dietary restriction and subsequent compensatory growth in cattle

 

RES

ADLIB

Significance

Geneb

Period 1

Period 2

Period 1

Period 2

SEMc

D

P

D*Pd

Growth and Structure

CCND1

5.804

16.219

7.981

11.04

2.701

0.586

0.018

0.182

CCND2

6.544

20.787

13.521

13.724

4.054

0.618

0.183

0.192

CCND3

6.156

50.062

9.498

37.621

9.179

0.957

<0.001

0.396

CCNE1

20.302

43.776

31.736

30.536

8.105

0.913

0.185

0.137

CCNE2

58.975

106.52

86.475

79.928

27.07

0.913

0.948

0.325

CDKN1A

11.429

21.017

13.367

13.944

4.724

0.590

0.288

0.347

CDKN2A

5.6178

14.964

12.357

17.614

3.236

0.245

0.039

0.532

CDKN2B

13.851

23.052

14.281

12.594

5.554

0.372

0.503

0.334

DSG1

7.82

50.432

40.351

27.469

13.02

0.273

0.051

0.019

VFA activation

ACS

2.72

2.359

2.12

2.588

0.155

0.826

0.103

0.733

ACSS1

2.579

2.047

2.703

2.079

0.338

0.818

0.096

0.893

PCCA

1.75

1.911

1.498

1.896

0.128

0.303

0.004

0.358

Transport proteins

CACT

6.597

8.145

10.829

4.969

1.61

0.745

0.189

0.027

NHE1

17.983

7.76

15.672

8.039

1.238

0.418

<0.001

0.302

NHE2

3.455

2.964

2.94

3.197

0.355

0.695

0.745

0.300

NHE3

2.609

8.503

2.759

6.313

0.79

0.212

<0.001

0.148

Ketogenesis

ACAT

12.153

31.857

12.779

20.89

4.802

0.289

0.006

0.235

HMGCL

2.313

5.241

3.169

5.171

0.42

0.356

<0.001

0.278

BDH1

3.971

1.67

3.29

1.99

0.251

0.476

<0.001

0.053

BDH2

3.491

2.803

2.1

2.931

0.304

0.045

0.815

0.017

HMGCS1

6.51

3.476

6.989

2.607

0.779

0.804

<0.001

0.392

HMGCS2

2.641

3.676

2.21

4.586

0.401

0.554

<0.001

0.103

Pyruvate metabolism

LDHa

1.915

1.719

1.609

1.789

0.092

0.207

0.931

0.048

LDHb

1.749

2.151

1.685

2.074

0.121

0.564

0.002

0.959

PC

2.001

2.928

2.409

2.986

0.247

0.355

0.004

0.484

PDHA1

2.074

1.248

1.619

1.265

0.093

0.024

<0.001

0.015

  1. aGene expression values were normalized to the reference gene after adjustment for efficiencies and interplate variation and converted to values relative to the greatest cycle threshold (Ct) within each data set
  2. b CCND1 cyclin D1, CCND2 cyclin D2, CCND3 cyclin D3, CCNE1 cyclin E1, CCNE2 cyclin E2, CDKN1A cyclin dependant kinase inhibitor 1A, CDKN2A cyclin dependant kinase inhibitor 2A, CDKN2B cyclin dependant kinase inhibitor 2B, DSG1 desmoglein 1, ACS acetyl coA synthetase, ACSS1 acyl coA synthetase short-chain family member 1, PCCA propionyl coA carboxylase α polypeptide, CACT solute carrier family 25, carnitine/acylcarnitine translocase member 20, NHE1 NA+/H+ exchanger isoform 1, NHE2 NA+/H+ exchanger isoform 2, NHE3 NA+/H+ exchanger isoform 3, ACAT acetyl coA acyltransferase, HMGCL 3-Hydroxymethyl-3-methylglutaryl-coA lyase, hydroxymethylglutaricaacidura, BDH1 3-Hydroxybutyrate dehydrogenase, type 1, BDH2 3-Hydroxybutyrate dehydrogenase, type 2, HMGCS1 3-Hydroxy-3-methylglutaryl-coA synthase 1, HMGCS2 3-Hydroxy-3-methylglutaryl-coA synthase 2, LDHa lactate dehydrogenase isoform a, LDHb lactate dehydrogenase isoform b, PC pyruvate carboxylase, PDHA1 pyruvate dehydrogenase lipoamide, α 1
  3. cSEM, standard error of the mean
  4. dD*P, diet × period interaction