Table 1 Sequences of oligonucleotide primers used for qRT-PCR
Gene namea | Accession number | Primer sequences | Amplicon size, bp |
|---|---|---|---|
Endogenous control | |||
RPLP0 | NM_001012682 | F: AGGGCGTCCGCAATGTT | 54 |
R: CGACGGTTGGGTAACCAATC | |||
GAPDH | NM_001034034 | F: GATTGTCAGCAATGCCTCCT | 135 |
R: CCATCCACAGTCTTCTGGGT | |||
ATP5B | NM_175796 | F: CCCTCAAGGAGACCATCAAA | 184 |
R: GGACACCATGGAGGATGAGT | |||
HPRT1 | NM_001034035 | F: GCCGACCTGTTGGATTACAT | 205 |
R: GCATTGTCTTCCCAGTGTCA | |||
B2M | NM_173893 | F: AGCGTCCTCCAAAGATTCAA | 156 |
R: ACAGGTCTGACTGCTCCGAT | |||
Cellular structure and growth | |||
CCND1 | NM_001046273 | F: GCACTTCCTCTCCAAGATGC | 204 |
R: GTCAGGCGGTGATAGGAGAG | |||
CCND2 | NM_001076372 | F: CCAGACCTTCATCGCTCTGT | 163 |
R: GATCTTTGCCAGGAGATCCA | |||
CCND3 | NM_001034709 | F: TCCAAGCTGCGCGAGACTAC | 178 |
R: GAGAGAGCCGGTGCAGAATC | |||
CCNE1 | XM_612960 | F: TTGACAGGACTGTGAGAAGC | 187 |
R: TTCAGTACAGGCAGTGGCGA | |||
CCNE2 | NM_001015665 | F: CTGCATTCTGAGTTGGAACC | 229 |
R: CTTGGAGCTTAGGAGCGTAG | |||
CDKN1A | NM_001098958 | F: GCAGACCAGCATGACAGATT | 205 |
R: GTATGTACAAGAGGAGGCGT | |||
CDKN2A | XM_868375 | F:GTGCGCCGGTTCTTGATTAC | 105 |
R: CCCATCATCATCACCCGCTG | |||
CDKN2B | NM_001075894 | F: GCGGTGGATTATCCTGGACA | 210 |
R: CATCATCATCACCTGGATCG | |||
DSG1 | NM_174045.1 | F: AGACAGAGAGCAATATGGCCAGT | 121 |
R: TTCACACTCTGCTGACATACCATCT | |||
VFA activation | |||
ACS | DQ489534 | F: GCTCTCACTGAGGAGCTCAAGAA | 64 |
R: AATCCGGTGTGGCAATGG | |||
ACSS1 | BC114698.1 | F: CCGATCAGGTCCTGGTAGTGA | 200 |
R: GAGCCATCACTTGGCACCTC | |||
PCCA | BC123876 | F: AGAATGGAAGATGCCCTGGAT | 70 |
R: CCTCTCGAAGCAATGCGATAT | |||
Transport Proteins | |||
CACT | NM_001077936.2 | F: TCACGCTCATGCGAGATGTT | 94 |
R: TTGACGCTCTTTCCCTCTGG | |||
NHE1 | NM_174833.2 | F: CCGTCACTGTGGTCCTGTAT | 88 |
R: CTCAGGAAGCCGAGGATGAT | |||
NHE2 | XM_604493 | F: TGCTCATCATGGTGGGACTT | 83 |
R: CACATCAGTCTTCATGGCCG | |||
NHE3 | AJ131764.1 | F: AGGTGCAGCTGGAGATCAT | 102 |
R: GACCTTGTTCTCGTCCAGGA | |||
Ketogenesis | |||
ACAT | NM_001034319.1 | F: AATAGGCAGTGTTCGTCGGG | 102 |
R: CATGGACTCCACCCCACAAG | |||
HMGCL | NM_001075132 | F: TGCAGATGGGAGTGAGTGTCA | 73 |
R: GACGCCCCCTGTGCATAG | |||
BDH1 | NM_001034600 | F: GACTGCCACCACTCCCTACAC | 59 |
R: TCCGCAGCCACCAGTAGTAGT | |||
BDH2 | NM_001034488 | F: CTGAAGAGGCACTGAGCGAT | 98 |
R: ATCAGAGGCCAAGTACACGC | |||
HMGCS1 | AY581197 | F: AGGATACTCATCACTTGGCCAACT | 67 |
R: CATGTTCCTTCGAAGAGGGAAT | |||
HMGCS2 | NM_001045883 | F: TCTGGCCCATCACTCTGCC | 126 |
R: AGTGGGGAGCCTGGAGAAGC | |||
Pyruvate Metabolism | |||
LDHa | BC146210 | F: GGCAAAGACTATAATGTGACAGCAA | 60 |
R: ACGTGCCCCAGCTGTGA | |||
LDHb | BC142006 | F: CCAACCCAGTGGATATTCTCACA | 66 |
R: TCACACGGTGCTTGGGTAATC | |||
PC | NM_177946 | F: CTCCCACCATCTGTCCTTTCC | 72 |
R: TTTATTTGGGCAGGAGATGAATACG | |||
PDHA1 | NM_001101046 | F: CAGTTTGCTACTGCTGATCCTGAA | 72 |
R: AGGTGGATCGTTGCAGTAAATGT | |||