Table 4 Markers of oxidative balance in bovine spermatozoa affected by four doses of lycopene (LYC), untreated vs. treated with ferrous ascorbate (FeAA)
From: Antioxidant efficiency of lycopene on oxidative stress - induced damage in bovine spermatozoa
Fractions | |||||||||
|---|---|---|---|---|---|---|---|---|---|
Fractions untreated with FeAA | Fractions treated with FeAA | ||||||||
Ctrl 1 (SC Ctrl) | 2 mmol/L LYC | 1 mmol/L LYC | 0.5 mmol/L LYC | 0.25 mmol/L LYC | Ctrl 2 (FeAA Ctrl) | 2 mmol/L LYC | 1 mmol/L LYC | 0.5 mmol/L LYC | 0.25 mmol/L LYC |
SOD, U/mg prot | |||||||||
0.43 ± 0.06 | 0.47 ± 0.02 | 0.45 ± 0.06 | 0.45 ± 0.07 | 0.44 ± 0.04 | 0.31 ± 0.05**1 | 0.43 ± 0.06**2 | 0.42 ± 0.07**2 | 0.39 ± 0.01 | 0.39 ± 0.01 |
CAT, U/mg prot | |||||||||
9.14 ± 0.86 | 11.75 ± 0.78 | 10.06 ± 1.05 | 10.68 ± 1.08 | 9.72 ± 1.04 | 4.85 ± 0.71***1 | 9.83 ± 0.57***2 | 9.68 ± 1.25***2 | 8.54 ± 0.58**2 | 8.23 ± 0.72 |
GPx, U/mg prot | |||||||||
0.088 ± 0.016 | 0.121 ± 0.019*1 | 0.119 ± 0.019*1 | 0.099 ± 0.006 | 0.090 ± 0.039 | 0.037 ± 0.010***1 | 0.066 ± 0.003*2 | 0.066 ± 0.016*2 | 0.058 ± 0.021 | 0.057 ± 0.004 |
GSH, mg/g prot | |||||||||
8.10 ± 1.02 | 11.14 ± 1.21 | 8.35 ± 1.80 | 8.87 ± 1.10 | 8.27 ± 1.15 | 5.51 ± 0.99*1 | 7.53 ± 0.84*2 | 7.53 ± 1.24*2 | 7.21 ± 0.69 | 6.72 ± 0.97 |
MDA, μmol/g prot | |||||||||
4.27 ± 0.24 | 3.09 ± 0.26 | 3.32 ± 0.33 | 3.33 ± 0.38 | 3.65 ± 0.38 | 10.25 ± 0.75***1 | 4.21 ± 0.25***2 | 4.35 ± 0.24***2 | 4.52 ± 0.14***2 | 4.73 ± 0.40***2 |