Skip to main content

Advertisement

Table 4 Markers of oxidative balance in bovine spermatozoa affected by four doses of lycopene (LYC), untreated vs. treated with ferrous ascorbate (FeAA)

From: Antioxidant efficiency of lycopene on oxidative stress - induced damage in bovine spermatozoa

Fractions
Fractions untreated with FeAA Fractions treated with FeAA
Ctrl 1 (SC Ctrl) 2 mmol/L LYC 1 mmol/L LYC 0.5 mmol/L LYC 0.25 mmol/L LYC Ctrl 2 (FeAA Ctrl) 2 mmol/L LYC 1 mmol/L LYC 0.5 mmol/L LYC 0.25 mmol/L LYC
SOD, U/mg prot
 0.43 ± 0.06 0.47 ± 0.02 0.45 ± 0.06 0.45 ± 0.07 0.44 ± 0.04 0.31 ± 0.05**1 0.43 ± 0.06**2 0.42 ± 0.07**2 0.39 ± 0.01 0.39 ± 0.01
CAT, U/mg prot
 9.14 ± 0.86 11.75 ± 0.78 10.06 ± 1.05 10.68 ± 1.08 9.72 ± 1.04 4.85 ± 0.71***1 9.83 ± 0.57***2 9.68 ± 1.25***2 8.54 ± 0.58**2 8.23 ± 0.72
GPx, U/mg prot
 0.088 ± 0.016 0.121 ± 0.019*1 0.119 ± 0.019*1 0.099 ± 0.006 0.090 ± 0.039 0.037 ± 0.010***1 0.066 ± 0.003*2 0.066 ± 0.016*2 0.058 ± 0.021 0.057 ± 0.004
GSH, mg/g prot
 8.10 ± 1.02 11.14 ± 1.21 8.35 ± 1.80 8.87 ± 1.10 8.27 ± 1.15 5.51 ± 0.99*1 7.53 ± 0.84*2 7.53 ± 1.24*2 7.21 ± 0.69 6.72 ± 0.97
MDA, μmol/g prot
 4.27 ± 0.24 3.09 ± 0.26 3.32 ± 0.33 3.33 ± 0.38 3.65 ± 0.38 10.25 ± 0.75***1 4.21 ± 0.25***2 4.35 ± 0.24***2 4.52 ± 0.14***2 4.73 ± 0.40***2
  1. Mean ± Standard Error
  2. SOD superoxide dismutase activity, CAT catalase activity, GPx glutathione peroxidase activity, GSH concentration of reduced glutathione, MDA malondialdehyde concentration
  3. *P < 0.05; **P < 0.01; ***P < 0.001
  4. 1 – vs. Control 1 (SC Control)
  5. 2 – vs. Control 2 (FeAA Control)