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Table 3 Reactive oxygen species (ROS) production by bovine spermatozoa [RLU/s/106 sperm] affected by four doses of lycopene (LYC), untreated vs. treated with ferrous ascorbate (FeAA)

From: Antioxidant efficiency of lycopene on oxidative stress - induced damage in bovine spermatozoa

Fractions
Fractions untreated with FeAA Fractions treated with FeAA
Ctrl 1 (SC Ctrl) 2 mmol/L LYC 1 mmol/L LYC 0.5 mmol/L LYC 0.25 mmol/L LYC Ctrl 2 (FeAA Ctrl) 2 mmol/L LYC 1 mmol/L LYC 0.5 mmol/L LYC 0.25 mmol/L LYC
Time 0 h
 1.63 ± 0.32 1.01 ± 0.22 1.21 ± 0.30 1.30 ± 0.16 1.57 ± 0.30 4.22 ± 0.70**1 2.00 ± 0.49*2 2.12 ± 0.41*2 2.55 ± 0.39 3.16 ± 0.58*1
Time 2 h
 4.49 ± 0.42 2.49 ± 0.72 2.58 ± 0.50 3.66 ± 0.19 4.10 ± 0.25 9.75 ± 0.85***1 5.28 ± 0.22**2 5.79 ± 0.45**2 7.90 ± 0.48*1 8.11 ± 0.65**1
Time 6 h
 10.92 ± 0.62 6.55 ± 0.21**1 7.01 ± 0.54**1 8.66 ± 0.52 9.15 ± 0.36 23.55 ± 1.51***1 12.33 ± 1.09***2 14.77 ± 0.80***2 17.00 ± 1.21*2, **1 20.25 ± 1.56***1
  1. Mean ± Standard Error
  2. *P < 0.05; **P < 0.01; ***P < 0.001
  3. 1 – vs. Control 1 (SC Control)
  4. 2 – vs. Control 2 (FeAA Control)