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Table 3 Reactive oxygen species (ROS) production by bovine spermatozoa [RLU/s/106 sperm] affected by four doses of lycopene (LYC), untreated vs. treated with ferrous ascorbate (FeAA)

From: Antioxidant efficiency of lycopene on oxidative stress - induced damage in bovine spermatozoa

Fractions

Fractions untreated with FeAA

Fractions treated with FeAA

Ctrl 1 (SC Ctrl)

2 mmol/L LYC

1 mmol/L LYC

0.5 mmol/L LYC

0.25 mmol/L LYC

Ctrl 2 (FeAA Ctrl)

2 mmol/L LYC

1 mmol/L LYC

0.5 mmol/L LYC

0.25 mmol/L LYC

Time 0 h

 1.63 ± 0.32

1.01 ± 0.22

1.21 ± 0.30

1.30 ± 0.16

1.57 ± 0.30

4.22 ± 0.70**1

2.00 ± 0.49*2

2.12 ± 0.41*2

2.55 ± 0.39

3.16 ± 0.58*1

Time 2 h

 4.49 ± 0.42

2.49 ± 0.72

2.58 ± 0.50

3.66 ± 0.19

4.10 ± 0.25

9.75 ± 0.85***1

5.28 ± 0.22**2

5.79 ± 0.45**2

7.90 ± 0.48*1

8.11 ± 0.65**1

Time 6 h

 10.92 ± 0.62

6.55 ± 0.21**1

7.01 ± 0.54**1

8.66 ± 0.52

9.15 ± 0.36

23.55 ± 1.51***1

12.33 ± 1.09***2

14.77 ± 0.80***2

17.00 ± 1.21*2, **1

20.25 ± 1.56***1

  1. Mean ± Standard Error
  2. *P < 0.05; **P < 0.01; ***P < 0.001
  3. 1 – vs. Control 1 (SC Control)
  4. 2 – vs. Control 2 (FeAA Control)