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Table 2 The Primers used for real-time PCR analysis

From: Effects of dietary protein restriction on muscle fiber characteristics and mTORC1 pathway in the skeletal muscle of growing-finishing pigs

Genes

Primer sequences (5’→3’)

Product size, bp

Accession NO.

MyHC I

F: GGCCCCTTCCAGCTTGA

63

L10129

R: TGGCTGCGCCTTGGTTT

  

MyHC IIa

F: TTAAAAAGCTCCAAGAACTGTTTCA

100

U11772

R: CCATTTCCTGGTCGGAACTC

  

MyHC IIx

F: AGCTTCAAGTTCTGCCCCACT

76

U90719

R: GGCTGCGGGTTATTGATGG

  

MyHC IIb

F:CACTTTAAGTAGTTGTCTGCCTTGAG

80

U90720

R: GGCAGCAGGGCACTAGATGT

  

MyoD

F: CAACAGCGGACGACTTCTATG

383

NM001002824

R: GCGCAAGATTTCCACCTT

  

MyoG

F: GCAGGGTGCTCCTCTTCA

230

NM001012406

R: AGGCTACGAGCGGACTGA

  

IL-6

R: AGTTGAAGGTGGTCTCGTGG

118

NM001252429

R: TCTGCCAGTACCTCCTTGCT

  

IL-15

F: GCATCCAGTGCTACTTGTGT

118

NM214390

R: TGCCAGGTTGCTTCTGTTTT

  

MuRF1

F:AGCACGAAGACGAGAAAATC

150

NM001184756

R:TGCGGTTACTCAGCTCAGTC

  

MAFbx

F:CCAGAGAGTCGGCAAGT

374

NM001044588

R:GAGGGTAGCATCGCACAAGT

  

β-actin

F: TGCGGGACATCAAGGAGAAG

216

XM003357928.2

R: AGTTGAAGGTGGTCTCGTGG

  
  1. MyHC myosin heavy chain, MyoD myoblast determination protein, MyoG myogenin, IL-6 interleukin-6, IL-15 interleukin-15, MuRF1 muscle ring finger 1, MAFbx muscle atrophy F-box