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Table 1 Amino acid composition of experimental treatments

From: Essential amino acid ratios and mTOR affect lipogenic gene networks and miRNA expression in bovine mammary epithelial cells

  Treatmentsa
  OPAARMC OPAA LT1.3 LT2.1 LH3.0 LV1.6
Experiment, μg/mL       
Lys 175 175 175 175 175 175
Met 60 60 60 60 60 60
Lys/Met 2.9:1 2.9:1 2.9:1 2.9:1 2.9:1 2.9:1
Thr 97 97 135 83 97 97
Phe 92 92 128 79 92 92
Thr/Phe 1.05:1 1.05:1 1.05:1 1.05:1 1.05:1 1.05:1
Lys/Thr 1.8:1 1.8:1 1.3:1 2.1:1 1.8:1 1.8:1
His 74 74 74 74 57 74
Lys/His 2.38:1 2.38:1 2.38:1 2.38:1 3.05:1b 2.38:1
Val 142 142 142 142 142 108
Lys/Val 1.23:1 1.23:1 1.23:1 1.23:1 1.23:1 1.62:1c
Rapamycin, ng/mL 9.147 - - - - -
Other amino acids, μg/mL       
Arg 84 84 84 84 84 84
Cys 63 63 63 63 63 63
Gln 584 584 584 584 584 584
Gly 30 30 30 30 30 30
Ile 105 105 105 105 105 105
Leu 105 105 105 105 105 105
Ser 42 42 42 42 42 42
Trp 16 16 16 16 16 16
Tyr 104 104 104 104 104 104
  1. aOPAARMC = optimal amino acid ratios with rapamycin (control); OPAA = optimal amino acid; LT2.1 = Lys:Thr at 2.1; LT1.3 = Lys:Thr at 1.3; LH3.0 = Lys:His at 3.05; LV1.6 = Lys:Val at 1.62. Amino acid ratios derived from Rulquin et al. [2]. Experimental treatments were designed by adding the required amount of the essential AA to the basal lactogenic essential AA-free media (HG-DMEM, Custom Media, Invitrogen) to reach the desired concentration
  2. bFrom Lee et al. [19]
  3. cFrom Haque et al. [22]