Journal of Animal Science and Biotechnology

Table 1 Amino acid composition of experimental treatments

From: Essential amino acid ratios and mTOR affect lipogenic gene networks and miRNA expression in bovine mammary epithelial cells

	Treatments^a
	OPAARMC	OPAA	LT1.3	LT2.1	LH3.0	LV1.6
Experiment, μg/mL
Lys	175	175	175	175	175	175
Met	60	60	60	60	60	60
Lys/Met	2.9:1	2.9:1	2.9:1	2.9:1	2.9:1	2.9:1
Thr	97	97	135	83	97	97
Phe	92	92	128	79	92	92
Thr/Phe	1.05:1	1.05:1	1.05:1	1.05:1	1.05:1	1.05:1
Lys/Thr	1.8:1	1.8:1	1.3:1	2.1:1	1.8:1	1.8:1
His	74	74	74	74	57	74
Lys/His	2.38:1	2.38:1	2.38:1	2.38:1	3.05:1^b	2.38:1
Val	142	142	142	142	142	108
Lys/Val	1.23:1	1.23:1	1.23:1	1.23:1	1.23:1	1.62:1^c
Rapamycin, ng/mL	9.147	-	-	-	-	-
Other amino acids, μg/mL
Arg	84	84	84	84	84	84
Cys	63	63	63	63	63	63
Gln	584	584	584	584	584	584
Gly	30	30	30	30	30	30
Ile	105	105	105	105	105	105
Leu	105	105	105	105	105	105
Ser	42	42	42	42	42	42
Trp	16	16	16	16	16	16
Tyr	104	104	104	104	104	104

^aOPAARMC = optimal amino acid ratios with rapamycin (control); OPAA = optimal amino acid; LT2.1 = Lys:Thr at 2.1; LT1.3 = Lys:Thr at 1.3; LH3.0 = Lys:His at 3.05; LV1.6 = Lys:Val at 1.62. Amino acid ratios derived from Rulquin et al. [2]. Experimental treatments were designed by adding the required amount of the essential AA to the basal lactogenic essential AA-free media (HG-DMEM, Custom Media, Invitrogen) to reach the desired concentration
^bFrom Lee et al. [19]
^cFrom Haque et al. [22]

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ISSN: 2049-1891

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