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Table 5 Validation of microarray gene expressions (IL-1β, IL-2 and IL-6) using Realtime-PCR method

From: High-concentrate feeding upregulates the expression of inflammation-related genes in the ruminal epithelium of dairy cattle

Gene symbol qRT-PCR relative expression Microarray expression
LC HC P-value fold change P value
IL-1β 1.15 ± 0.164 2.40 ± 0.159 P < 0.05 +1.58 P < 0.05
IL-2 0.80 ± 0.142 1.58 ± 0.135 P < 0.05 +2.20 P < 0.05
IL-6 1.31 ± 0.010 0.66 ± 0.068 P < 0.05 - 1.76 P < 0.05