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Table 5 Validation of microarray gene expressions (IL-1β, IL-2 and IL-6) using Realtime-PCR method

From: High-concentrate feeding upregulates the expression of inflammation-related genes in the ruminal epithelium of dairy cattle

Gene symbol

qRT-PCR relative expression

Microarray expression

LC

HC

P-value

fold change

P value

IL-1β

1.15 ± 0.164

2.40 ± 0.159

P < 0.05

+1.58

P < 0.05

IL-2

0.80 ± 0.142

1.58 ± 0.135

P < 0.05

+2.20

P < 0.05

IL-6

1.31 ± 0.010

0.66 ± 0.068

P < 0.05

- 1.76

P < 0.05