High-concentrate feeding upregulates the expression of inflammation-related genes in the ruminal epithelium of dairy cattle

Table 1 Primers for real-time quantitative PCR

Gene Name	Gene ID	Primer sequence (5′ → 3′)	Amplicon Size,bp	Reference
IL-1β	NM_174093.1	For: AACCGAGAAGTGGTGTTCTGC	167	[18]
		R: TTGGGGTAGACTTTGGGGTCT
IL-2	NM_180997.2	For: ACATTTGACTTTTACGCGCCCAAG	307	[19]
		R: AATGAGAGGCACTTAGTGATC
IL-6	NM_000600.3	For: GGAGGAAAAGGACGGATGCT	227	[18]
		R: GGTCAGTGTTTGTGGCTGGA
IL-8	NM_173925	For: CCTCTTGTTCAATATGACTTCCA	170	[18]
		R: GGCCCACTCTCAATAACTCTC
IL-12β	U11815	For: AGGTCGTGGTAGAAGCTGTG	276	[20]
		R: CCTTGTGGCATGTGACTTTG
TNF-α	NM_173966.2	For: CTTCTGCCTGCTGCACTTCG	156	[18]
		R: GAGTTGATGTCGGCTACAACG
GAPDH	NM001034034	For: GGGTCATCATCTCTGCACCT	176	[21]
		R: GGTCATAAGTCCCTCCACGA

ISSN: 2049-1891