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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Effect of in ovo folic acid injection on hepatic IGF2 expression and embryo growth of broilers

Fig. 2

Methylation analysis of the IGF2 promoter region in the liver. a Map of CpG dinucleotide in IGF2 promoter regions are presented by the ratable line. Numbers under the line are the positions relative to the transcription start site (TSS). b The methylation patterns of each CpG site were shown as maps of empty and full circles. Empty circles showed unmethylated CpGs and full circles methylated CpGs. Each vertical line is made up of four circles and represent one bacterial clone. c The total methylation and each CpG site methylation level of promoter region were calculated by analyzing the 39 clones in total. The asterisk (*) indicated statistically significant differences (P < 0.05). The P values for linear and quadratic regression analysis were > 0.05

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