Fig. 4

Identification of exogenous TLR4 gene and quantification. a The brief structure of 3S-pTLR4 vector, the sizes of the injected TLR4 fragment was 3172 bp. b Southern blot analysis. The 5118 band was the endogenous TLR4 fragment when the sheep genomic DNA was digest by Hind III. Numbered 1, 2, 3, 5 and 7 represent positive individuals. Numbered 4, 6 and 8 represent negative individuals. 2× and 4× are samples of transgenic vectors, here used as positive controls. c The mRNA expression of TLR4 was quantified using real-time PCR, each individual was repeated three times (n = 3). Tg = Transgenic sheep, N = 5. NTg = Non-transgenic sheep, N = 10. Superscript letter (*) represents statistically significant difference (P < 0.05)