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Table 5 Effect of h[Gly2]GLP-21-34 on enzyme activity in the small intestine of LPS-challenged weaned pigletsa

From: The prolonged effect of glucagon-like peptide 2 pretreatment on growth performance and intestinal development of weaned piglets

Item

Treatmentb

Pooled SEM

Contrastc

 

Control

LPS

LPS + Low GLP-2

LPS + High GLP-2

1

2

3

Duodenum

Na+-K+-ATPase, U/mg protein

541

511

536

522

18

0.573

0.636

0.828

AKP, U/mg protein

47.6

27.3

42.1

49.6

1.8

<0.001

<0.001

<0.001

γ-GT, U/g protein

1,035

872

1,015

1,117

30

0.045

0.079

0.004

Amylase, U/mg protein

74.5

67.6

74.3

76.3

1.3

0.060

0.068

0.019

Lipase, U/mg protein

17,777

15,227

17,792

19,608

435

0.020

0.019

<0.001

Jejunum

Na+-K+-ATPase, U/mg protein

566

524

524

532

13

0.275

0.996

0.829

AKP, U/mg protein

15.4

12.4

14.4

16.5

0.5

0.047

0.178

0.007

γ-GT, U/g protein

988

818

945

1,013

26

0.019

0.075

0.008

Amylase, U/mg protein

154

144

166

161

4.7

0.467

0.103

0.215

Lipase, U/mg protein

48,436

38,616

44,289

52,209

1695

0.031

0.204

0.004

  1. aGLP-2 = Glucagon-like peptide 2; LPS = Escherichia coli lipopolysaccharide; AKP = Alkaline phosphatase; γ-GT = γ-Glutamyltranspeptidase
  2. bControl: piglets injected with sterile saline on d 14 postweaning; LPS: pigs challenged by LPS on d 14 postweaning; LPS + Low and LPS + High GLP-2: piglets pretreated with GLP-2 at doses of 2 and 10 nmol/kg BW per day for the first 7 d after weaning were challenged by LPS on d 14 postweaning. The data were obtained 24 h after injection with LPS and 15 d post-weaning
  3. cContrast: (1) control v. LPS; (2) LPS v. LPS + Low GLP-2; (3) LPS v. LPS + High GLP-2; Values are means of ten replicates