Table 1 Primer sequences for PCR of porcine adipokines
From: Regulation of cytokine gene expression by orosomucoid in neonatal swine adipose tissue
Gene | Primer | Primer Sequence | Amplicon Size, bp | Amplification Efficiency, % | GenBank Accession No. |
|---|---|---|---|---|---|
Tumor Necrosis Factor α | Sense | 5'-CCCCTCTGAAAAAGACACCA-3' | 180 | 106 | NM214022 |
Antisense | 5'-TCGAAGTGCAGTAGGCAGAA-3' | ||||
Interleukin 6 | Sense | 5'-ATGGCAGAAAAAGACGGATG-3' | 215 | 98 | NM214399 |
Antisense | 5'-GTGGTGGCTTTGTCTGGATT-3' | ||||
MCP (CCL2) | Sense | 5'-TCTCCAGTCACCTGCTGCTA-3' | 210 | 95 | NM214214 |
Antisense | 5'-AGGCTTCGGAGTTTGGTTTT-3' | ||||
MIF | Sense | 5'-CGATGTTCGTGGTAAACACC-3' | 235 | 101 | DQ989235 |
Antisense | 5'-AGCAGCTTGCTGTAGGAACG-3' | ||||
Adiponectin | Sense | 5'-TACCCCAGGCCGTGATGGCA-3' | 213 | 92 | AY135647 |
Antisense | 5'-ACCCGAGTCTCCAGGCCCAC-3' | ||||
Cyclophilin | Sense | 5'-ATGGTAACCCCACCGTCTTC-3' | 376 | 99 | AY008846 |
Antisense | 5'-GTTTGCCATCCAACCACTCAG-3' |