Effects of thymol and carvacrol supplementation on intestinal integrity and immune responses of broiler chickens challenged with Clostridium perfringens

Table 3 RT-PCR primers and Genbank accession numbers of chicken

Target	Primer sequence	Accession no.	Product size, bp
Target	(5′–3′)^a	Accession no.	Product size, bp
TLR2	F: GATTGTGGACAACATCATTGACTC	NM_001161650	294
	R: AGAGCTGCTTTCAAGTTTTCCC	NM_001161650	294
TLR4	F: AGTCTGAAATTGCTGAGCTCAAAT	NM_001030693	190
	R: GCGACGTTAAGCCATGGAAG	NM_001030693	190
IL-1β	F: ACTGGGCATCAAGGGCTA	NM_204524	131
	R: GGTAGAAGATGAAGCGGGTC	NM_204524	131
TNF-α	F: GAGCGTTGACTTGGCTGTC	NM_204267	64
TNF-α	R: AAGCAACAACCAGCTATGCAC	NM_204267	64
Claudin-1	F: CATACTCCTGGGTCTGGTTGGT	AY750897.1	100
Claudin-1	R: GACAGCCATCCGCATCTTCT	AY750897.1	100
Occludin	F: ACGGCAGCACCTACCTCAA	D21837.1	123
Occludin	R: GGGCGAAGAAGCAGATGAG	D21837.1	123
Mucin-2	F: TTCATGATGCCTGCTCTTGTG	XM_421035	93
Mucin-2	R: CCTGAGCCTTGGTACATTCTTGT	XM_421035	93
β-actin	F: GAGAAATTGTGCGTGACATCA	L08165	152
β-actin	R: CCTGAACCTCTCATTGCCA	L08165	152

ISSN: 2049-1891