Effects of thymol and carvacrol supplementation on intestinal integrity and immune responses of broiler chickens challenged with Clostridium perfringens

Table 2 Diet composition and nutrient levels

Items (%, unless otherwise indicated)	Starter diets (d 0–21)	Grower diets (d 22–28)
Ingredient
Wheat	62.75	68.50
Soybean meal	29.61	23.72
Soybean oil	3.40	4.00
Dicalcium phosphate	1.91	1.63
Limestone	1.04	0.96
Sodium chloride	0.35	0.35
Choline chloride (50 %)	0.25	0.25
L-Lysine (99 %)	0.25	0.24
DL-Methionine (98 %)	0.19	0.11
Santoquin	0.03	0.03
Trace mineral premix^a	0.20	0.20
Vitamin premix^b	0.02	0.02
Calculated nutrient level
ME (Mcal/kg)	2.90	2.98
Protein	21.00	19.00
Calcium	1.00	0.90
Available Phosphorus	0.45	0.40
Lysine	1.15	1.00
Methionine	0.50	0.40

^aThe trace mineral premix provided the following (per kg of diet): manganese, 100 mg; zinc, 75 mg; iron, 80 mg; copper, 8 mg; selenium, 0.25 mg; iodine, 0.35 mg
^bThe vitamin premix provided the following (per kg of diet): vitamin A, 18,750 IU; vitamin D₃, 3750 IU; vitamin E, 28 IU; vitamin K₃, 3.975 mg; thiamine mononitrate, 3 mg; riboflavin, 9 mg; vitamin B₁₂, 0.0375 mg; d-biotin, 0.150 mg; folic acid, 1.875 mg; d-calcium pantothenate, 18 mg; nicotinic acid, 75 mg

ISSN: 2049-1891