Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows

Table 3 Expression of target genes in PMN isolated on -10, +3, and 21 d around parturition in Holstein cows fed a lower-energy control diet (CON), a higher-energy diet (OVE) or OVE plus Smartamine M (OVE + SM) during the close-up period and through the first 30 d postpartum

Gene	Treatment^a			SEM^b	P-value
Gene	CON	OVE	OVE + SM	SEM^b	Diet	Time	D × T^c
Met and glutathione metabolism
AHCY	5.23^e	13.47^d	12.23^d	2.47	0.02	0.67	0.10
GSR	5.76	9.21	10.12	2.02	0.28	0.34	0.06
GPX1	9.82	9.02	7.66	0.94	0.24	0.04	0.05
Inflammation
NFKB1	7.80^e	17.58^d	19.24^d	3.60	<0.01	0.80	0.98
STAT3	8.37	11.40	10.46	1.80	0.48	0.87	0.07
TLR4	−4.28^e	−3.20^d,e	−2.94^d	0.57	0.07	0.96	0.06
TNF	−3.34^e	5.91^d	6.36^d	2.05	<0.01	0.66	0.77
LTA4H	0.08	0.06	0.05	0.01	0.26	0.46	0.01
RXRA	11.32	12.94	13.39	1.59	0.61	0.11	0.03
Cellular receptors
SELL	2.38	5.04	4.93	1.23	0.23	0.62	<0.01
ITGAM	5.04^e	10.69^d	10.37^d	1.73	0.04	0.07	0.07
TLN1	12.53	9.77	10.08	1.11	0.18	0.24	0.07
VCL	−5.00^e	−3.98^d	−4.24^d	0.26	0.02	<0.01	<0.01
Oxidative stress
SOD1	7.09	9.93	10.31	1.98	0.34	0.36	0.10
SOD2	−0.68^e	−0.83^e	0.21^d	0.32	0.06	0.04	0.08
S100A8	8.88	7.28	6.96	0.88	0.25	0.25	0.04

^aCON = lower-energy control (n = 9; 1.24 Mcal/kg DM); OVE = higher-energy (n = 9; 1.54 Mcal/kg DM); OVE + SM = higher-energy plus Smartamine M (n = 10; SM = 0.07 % of DM)
^bLargest SEM is shown
^c Interaction between diet and time
^d-eMean values within a row with different superscripts were significantly different (P < 0.05)

ISSN: 2049-1891