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Table 1 Ingredient and chemical composition of diets

From: Peripartal rumen-protected methionine supplementation to higher energy diets elicits positive effects on blood neutrophil gene networks, performance and liver lipid content in dairy cows

 

Close-up perioda

Lactationb

Item

CON

OVE

OVE + SM

Ingredient, % of DM

 Alfalfa silage

12.00

8.20

8.20

5.00

 Alfalfa hay

—

3.50

3.50

4.00

 Corn silage

33.00

35.90

35.90

33.00

 Wheat straw

36.00

15.40

15.40

4.00

 Cottonseed

—

—

—

3.50

 Wet brewers grains

—

6.00

6.00

10.00

 Ground shelled corn

4.00

13.00

13.00

22.20

 Soy hulls

2.00

4.00

4.00

4.00

 Soybean meal, 48 % CP

7.94

3.10

3.10

3.30

 Expeller soybean mealc

—

2.00

2.00

6.20

 SoyChlord

0.15

3.80

3.80

—

 Blood meal, 85 % CP

1.00

1.00

1.00

0.30

 Smartamine Me

—

—

0.07

—

 Urea

0.45

0.30

0.30

0.14

 Rumen-inert fatf

—

—

—

1.00

 Limestone

1.30

1.30

1.30

1.18

 Salt (plain)

0.32

0.30

0.30

0.27

 Dicalcium phosphate

0.12

0.18

0.18

0.27

 Magnesium oxide

0.21

0.08

0.08

0.14

 Magnesium sulfate

0.91

0.97

0.97

—

 Sodium bicarbonate

—

—

—

0.75

 Potassium carbonate

—

—

—

0.10

 Calcium sulfate

—

—

—

0.10

 Mineral-vitamin mixg

0.20

0.20

0.20

0.20

 Vitamin Ah

0.015

0.015

0.015

—

 Vitamin Di

0.025

0.025

0.025

—

 Vitamin Ej

0.38

0.38

0.38

—

 Biotin

—

0.35

0.35

0.35

 DMk, %

46.6 ± 0.8

45.2 ± 0.8

45.2 ± 0.8

45.2 ± 1.5

Chemical analysis, %

 NEL, Mcal/kg

1.24

1.47

1.47

1.65

 CP, % of DM

14.6

15.6

15.6

16.3

 ADF, % of DM

36.2

30.2

30.2

24.1

 NDF, % of DM

52.7

44.7

44.7

37.9

  1. aThe control diet (CON) was fed to all cows during the far-off dry period (−50 to −21 d relative to expected calving). During the close-up period (−21 d to calving) cows were assigned either to a higher-energy diet (OVE), OVE plus Smartamine M (OVE + SM) or continuously fed the CON diet
  2. bAll cows received the same lactation diet; however, Smartamine M (0.07 % of DMI) supplementation to the OVE + SM diet continued until 30 DIM
  3. cSoyPLUS (West Central Soy, Ralston, IA, USA)
  4. dSoyChlor (West Central Soy, Ralston, IA, USA)
  5. eSmartamine M (Adisseo NA, Alpharetta, GA, USA)
  6. fEnergy Booster 100 (MSC, Carpentersville, IL, USA)
  7. gContained a minimum of 5 % Mg, 10 % S, 7.5 % K, 2.0 % Fe, 3.0 % Zn, 3.0 % Mn, 5000 mg of Cu/kg, 250 mg of I/kg, 40 mg of Co/kg, 150 mg of Se/kg, 2200 kIU of vitamin A/kg, 660 kIU of vitamin D3/kg, and 7700 IU of vitamin E/kg
  8. hContained 30,000 kIU/kg
  9. iContained 5009 kIU/kg
  10. jContained 44,000 IU/kg
  11. kMeans ± SD