Table 2 Summary of studies describing iPSC generation in the selected farm animal species
Species | Donor cell type | Insertion method | Transcription factorsa | Culture conditions (growth surface; serum or serum replacements, factors/inhibitors)b | Reference |
|---|---|---|---|---|---|
Pig | embryonic fibroblast | retroviral transduction | hOSKM and mOSKM | iMEFs; defined FBS + bFGF | [51] |
fetal fibroblasts | lentiviral transduction | hOSKM | iMEFs; KOSR + bFGF | [52] | |
ear fibroblasts and bone marrow cells | Tet-on-inducible lentiviral transduction | hOSKMNL | iMEFs; KOSR | [53] | |
mesenchymal stem cells | lentiviral transduction | hOSKMNL | iMEFs; KOSR + bFGF | [45] | |
ear fibroblasts | retroviral transduction | hOSKM | iMEFs or gelatin; FBS and KOSR (1:1) + bFGF + LIF | [54] | |
ear fibroblasts | retroviral transduction | mSKM | iMEFs or gelatin; FBS and KOSR (1:1) + bFGF + LIF | [55] | |
embryonic fibroblasts | Tet-on-inducible lentiviral transduction | hOSKMN | iMEFs; KOSR + bFGF | [56] | |
fetal fibroblasts | lentiviral transduction | hOSKM | iMEFs; KOSR + bFGF | [57] | |
fetal fibroblasts | sleeping beauty transposon system | mOSKM | iMEFs or iSNLs or gelatin; KOSR + bFGF | [58] | |
embryonic fibroblasts | retroviral transduction | hOSKM | iMEFs; FBS and KOSR (1:1) + bFGF + LIF | [59] | |
embryonic fibroblasts | retroviral transduction | mOSKM | iMEFs; KOSR + bFGF or FBS + bFGF + LIF or FBS + bFGF + LIF + VPA | [60] | |
mesenchymal stem cells | retroviral transduction | pOK + small molecules | iMEFs; KOSR or FBS + LIF | [61] | |
fetal fibroblasts | episomal plasmid | hOSKMNL | iMEFs; N-2 suppl. + B-27 suppl. + LIF + GSKi + MEKi | [44] | |
Horse | fetal fibroblasts | piggyBac transposon system | mOSKM | 50 % iMEFs and 50 % iEFFs; FBS + bFGF + LIF + GSKi + TGFi + TZV + ALKi | [49] |
fibroblasts | retroviral transduction | hOSK | iMEFs; FBS + ITS + EGF + bFGF + LIF | [62] | |
skin fibroblasts | retroviral transduction | mOSKM | iSNLs; FBS or KOSR + bFGF and/or LIF | [63] | |
keratinocytes | retroviral transduction | mOSKM | iSNLs; FBS + bFGF + LIF | [64] | |
skin fibroblasts | lentiviral transduction | hOSKM | - reprogramming: Matrigel; ES-FCS + bFGF + LIF + GSKi + MEKi + TGFi + ALKi | [65] | |
- putative iPSCs: iMEFs; ES-FCS + LIF or ES-FCS + LIF + bFGF or ES-FCS + LIF + bFGF + MEKi or ES-FCS + LIF + bFGF + PI3K/AKTi or ES-FCS + LIF + bFGF + MEKi + PI3K/AKTi | |||||
Rabbit | liver and stomach cells | lentiviral transduction | hOSKM | iMEFs; KOSR + bFGF + LIF | [66] |
ear fibroblasts | retroviral transduction | hOSKM | iMEFs; KOSR + bFGF | [67] | |
Sheep | embryonic fibroblasts | retroviral transduction | hOSKM | iMEFs; FBS + ITS + bFGF + LIF | [68] |
ear fibroblasts | Tet-on-inducible lentiviral transduction | hOSKMNL + SV40 T + hTERT | iMEFs; KOSR | [69] | |
fetal fibroblasts | Tet-on-inducible lentiviral transduction | mOSKM | iMEFs; KOSR or FBS + bFGF | [70] | |
embryonic fibroblasts | retroviral transduction | mOSKM | iSNLs; KOSR + bFGF | [71] | |
Cattle | fetal fibroblasts | retroviral transduction | bOSKMNL | iMEFs; KOSR + bFGF | [72] |
fetal fibroblasts | lentiviral transduction | hOpSMK | iMEFs; FBS + bFGF + LIF | [73] | |
embryonic fibroblasts | poly-promoter plasmid | bOSKM | iMEFs; LIF + MEKi + GSKi | [74] | |
ear fibroblasts | retroviral transduction | hOSKMN | iMEFs; FBS + ITS + bFGF + LIF | [75] | |
fetal fibroblasts | piggyBac transposon systems | hOSKMNL | iMEFs; KOSR + bFGF + LIF | [76] | |
Goat | ear fibroblasts | Tet-on-inducible lentiviral transduction | hOSKMNL + SV40 T + hTERT | ND | [77] |
fetal ear fibroblasts | lentiviral transduction | hOSKM | iMEFs; KOSR + bFGF | [78] | |
fetal fibroblasts | lentiviral transduction | bOSKMNL + miR- 302/367 | - iMEFs; KOSR + LIF + MEKi + GSKi + VPA or KOSR + bFGF + VPA- iSNLs; KOSR + bFGF + VPA | [79] |