Skip to main content

Advertisement

Table 2 Summary of studies describing iPSC generation in the selected farm animal species

From: Cellular reprogramming in farm animals: an overview of iPSC generation in the mammalian farm animal species

Species Donor cell type Insertion method Transcription factorsa Culture conditions (growth surface; serum or serum replacements, factors/inhibitors)b Reference
Pig embryonic fibroblast retroviral transduction hOSKM and mOSKM iMEFs; defined FBS + bFGF [51]
fetal fibroblasts lentiviral transduction hOSKM iMEFs; KOSR + bFGF [52]
ear fibroblasts and bone marrow cells Tet-on-inducible lentiviral transduction hOSKMNL iMEFs; KOSR [53]
mesenchymal stem cells lentiviral transduction hOSKMNL iMEFs; KOSR + bFGF [45]
ear fibroblasts retroviral transduction hOSKM iMEFs or gelatin; FBS and KOSR (1:1) + bFGF + LIF [54]
ear fibroblasts retroviral transduction mSKM iMEFs or gelatin; FBS and KOSR (1:1) + bFGF + LIF [55]
embryonic fibroblasts Tet-on-inducible lentiviral transduction hOSKMN iMEFs; KOSR + bFGF [56]
fetal fibroblasts lentiviral transduction hOSKM iMEFs; KOSR + bFGF [57]
fetal fibroblasts sleeping beauty transposon system mOSKM iMEFs or iSNLs or gelatin; KOSR + bFGF [58]
embryonic fibroblasts retroviral transduction hOSKM iMEFs; FBS and KOSR (1:1) + bFGF + LIF [59]
embryonic fibroblasts retroviral transduction mOSKM iMEFs; KOSR + bFGF or FBS + bFGF + LIF or FBS + bFGF + LIF + VPA [60]
mesenchymal stem cells retroviral transduction pOK + small molecules iMEFs; KOSR or FBS + LIF [61]
  fetal fibroblasts episomal plasmid hOSKMNL iMEFs; N-2 suppl. + B-27 suppl. + LIF + GSKi + MEKi [44]
Horse fetal fibroblasts piggyBac transposon system mOSKM 50 % iMEFs and 50 % iEFFs; FBS + bFGF + LIF + GSKi + TGFi + TZV + ALKi [49]
fibroblasts retroviral transduction hOSK iMEFs; FBS + ITS + EGF + bFGF + LIF [62]
skin fibroblasts retroviral transduction mOSKM iSNLs; FBS or KOSR + bFGF and/or LIF [63]
keratinocytes retroviral transduction mOSKM iSNLs; FBS + bFGF + LIF [64]
skin fibroblasts lentiviral transduction hOSKM - reprogramming: Matrigel; ES-FCS + bFGF + LIF + GSKi + MEKi + TGFi + ALKi [65]
- putative iPSCs: iMEFs; ES-FCS + LIF or ES-FCS + LIF + bFGF or ES-FCS + LIF + bFGF + MEKi or ES-FCS + LIF + bFGF + PI3K/AKTi or ES-FCS + LIF + bFGF + MEKi + PI3K/AKTi
Rabbit liver and stomach cells lentiviral transduction hOSKM iMEFs; KOSR + bFGF + LIF [66]
ear fibroblasts retroviral transduction hOSKM iMEFs; KOSR + bFGF [67]
Sheep embryonic fibroblasts retroviral transduction hOSKM iMEFs; FBS + ITS + bFGF + LIF [68]
ear fibroblasts Tet-on-inducible lentiviral transduction hOSKMNL + SV40 T + hTERT iMEFs; KOSR [69]
fetal fibroblasts Tet-on-inducible lentiviral transduction mOSKM iMEFs; KOSR or FBS + bFGF [70]
embryonic fibroblasts retroviral transduction mOSKM iSNLs; KOSR + bFGF [71]
Cattle fetal fibroblasts retroviral transduction bOSKMNL iMEFs; KOSR + bFGF [72]
fetal fibroblasts lentiviral transduction hOpSMK iMEFs; FBS + bFGF + LIF [73]
embryonic fibroblasts poly-promoter plasmid bOSKM iMEFs; LIF + MEKi + GSKi [74]
ear fibroblasts retroviral transduction hOSKMN iMEFs; FBS + ITS + bFGF + LIF [75]
fetal fibroblasts piggyBac transposon systems hOSKMNL iMEFs; KOSR + bFGF + LIF [76]
Goat ear fibroblasts Tet-on-inducible lentiviral transduction hOSKMNL + SV40 T + hTERT ND [77]
fetal ear fibroblasts lentiviral transduction hOSKM iMEFs; KOSR + bFGF [78]
fetal fibroblasts lentiviral transduction bOSKMNL + miR- 302/367 - iMEFs; KOSR + LIF + MEKi + GSKi + VPA or KOSR + bFGF + VPA- iSNLs; KOSR + bFGF + VPA [79]
  1. aO OCT4, S SOX2, K KLF4, M MYC, L LIN28, N NANOG, SV40 T Simian vacuolating virus 40 large T antigen, TERT telomerase reverse transcriptase, miR-302/367 microRNA cluster 302/367, h human, m mouse, p pig, b bovine
  2. bfor a detailed medium composition and culture conditions see reference – only growth surface/feeder layer and the selected growth medium supplements (serum/serum replacements, growth factors, and signalling pathway inhibitors) are presented in the table. iMEFs inactivated (mitomycin C) or irradiated mouse embryonic fibroblasts, FBS fetal bovine serum, bFGF basic fibroblast growth factor, KOSR knockout serum replacement, LIF leukemia inhibitory factor, iSNLs inactivated transformed mouse fibroblasts with expression of leukemia inhibitory factor, VPA valproic acid, iEFFs inactivated equine fetal fibroblasts, GSKi glycogen synthase kinase inhibitor, MEKi mitogen-activated protein kinase kinase 1 inhibitor, TGFi transforming growth factor-beta inhibitor, TZV thiazovivin, ALKi anaplastic lymphoma kinase inhibitor, ITS insulin/transferrin/selenium supplement, EGF epidermal growth factor, ES-FCS embryonic stem cells-qualified fetal calf serum, PI3K/AKTi phosphatidylinositol 3-kinase/protein B kinase (AKT) inhibitor, ND no data